Method for extracting cholest-3,5-dien-7-one from Aspergillus niger by separation

A technology of Aspergillus niger and cholesteric, which is applied in the field of extracting cholestan-3,5-dien-7-one, can solve the problems of no separation and extraction, and achieve the effects of reliable source, easy cultivation and preservation, and wide application range

Inactive Publication Date: 2018-10-16
GUIZHOU UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that Chen Yanchao et al. have detected cholesta-3,5-dien-7-one from clam oil by GC-MS method, but there is no information on the isolation and extraction of cholesta-3,5-dien-7-one from Aspergillus niger. Related research on en-7-one

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting cholest-3,5-dien-7-one from Aspergillus niger by separation
  • Method for extracting cholest-3,5-dien-7-one from Aspergillus niger by separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: A method for separating and extracting cholesta-3,5-dien-7-one by using Aspergillus niger, the steps are as follows:

[0034] 1) Preparation of extract: put the fermented mycelia of Aspergillus niger in a blast drying oven, bake at 55°C until constant weight, grind the powder, pass through a 60-mesh sieve to obtain the fungal powder, and use acetone to mix the fungal powder with a ratio of 1:4. Weight ratio, at a temperature of 75°C and a pressure of 0.5MPa, extract 3 times to obtain the Aspergillus niger extract, and the time is 3 hours each time, and the extract is rotated at a temperature of 50°C and a pressure of 0.05MPa using a rotary evaporator. Evaporate until the organic solvent evaporates to dryness to obtain the extract;

[0035] 2) Silica gel column chromatography: use petroleum ether to wet-pack the column to obtain a silica gel chromatography column, dissolve the extract in step (1) with acetone solvent and mix the sample with silica gel with ...

Embodiment 2

[0039] Example 2: A method for separating and extracting cholesta-3,5-dien-7-one by using Aspergillus niger, the steps are as follows:

[0040]1) Preparation of extract: put the fermented mycelia of Aspergillus niger in a blast drying oven, bake at 55°C until constant weight, grind the powder, pass through a 60-mesh sieve to obtain the fungal powder, and use acetone to mix the fungal powder with a ratio of 1:4. Weight ratio, extract 3 times at a temperature of 50°C and a pressure of 0MPa to obtain an Aspergillus niger extract, each time for 3h, and use a rotary evaporator to rotate the extract at a temperature of 40°C and a pressure of 0.04MPa When the organic solvent is evaporated to dryness, the extract is obtained;

[0041] 2) Silica gel column chromatography: use petroleum ether to wet-pack the column to obtain a silica gel chromatography column, dissolve the extract in step (1) with acetone solvent and mix the sample with silica gel with a size of 200 mesh, evaporate the ...

Embodiment 3

[0045] Example 3: A method for separating and extracting cholesta-3,5-dien-7-one using Aspergillus niger, the steps are as follows:

[0046] 1) Preparation of extract: put the fermented mycelia of Aspergillus niger in a blast drying oven, bake at 55°C until constant weight, grind the powder, pass through a 60-mesh sieve to obtain the fungal powder, and use acetone to mix the fungal powder with a ratio of 1:4. Weight ratio, at a temperature of 100°C and a pressure of 1.0MPa, extract 3 times to obtain the Aspergillus niger extract, and the time is 3 hours each time, and the extract is rotated at a temperature of 60°C and a pressure of 0.06MPa using a rotary evaporator. Evaporate until the organic solvent evaporates to dryness to obtain the extract;

[0047] 2) Silica gel column chromatography: use petroleum ether to wet-pack the column to obtain a silica gel chromatography column, dissolve the extract in step (1) with acetone solvent and mix the sample with silica gel with a siz...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting cholest-3,5-dien-7-one from Aspergillus niger by separation. The method comprises the following steps: fermented mycelium of Aspergillus niger are driedand ground, bacterial powder is obtained and subjected to extraction with acetone, an Aspergillus niger extract solution is prepared and concentrated, Aspergillus niger extract is prepared and subjected to chromatography, and cholest-3,5-dien-7-one is prepared, wherein Aspergillus niger is named xj (Aspergillus niger xj) with the preservation number of CCTCC NO:M 206021. Prepared cholest-3,5-dien-7-one P is biologically sourced and has the characteristics of cheap and easily available substrate, simple and feasible extraction process, environment-friendly production process, few solvent residues, low pollution and easily obtained culture medium.

Description

technical field [0001] The invention relates to a method for extracting cholesta-3,5-dien-7-one, in particular to a method for separating and extracting cholesta-3,5-dien-7-one by using Aspergillus niger. Background technique [0002] Aspergillus niger is a common species of fungi, which can survive in a wide temperature and pH range and low humidity, and is widely distributed in food, plant products and soils all over the world. As a generally recognized as safe (GRAS) strain certified by the U.S. Food and Drug Administration (U.S.FDA), Aspergillus niger is widely used in the industrial production of organic acids and enzymes, and has huge potential market value. Aspergillus niger produces citric acid, gluconic acid, gallic acid, oxalic acid, etc., and the main enzymes are amylase, catalase, cellulase, pectinase, and acid protease. Not only that, many metabolites of Aspergillus niger have various important application values. [0003] Cholesta-3,5-dien-7-one, the molecula...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 李祝张素冉江肖洋唐婧红张钦语
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap