Wheat Seed Dormancy Persistence Gene tacngc-2a and Its Functional Markers

Abstract

The invention belongs to the field of crop genetics and breeding, and provides a wheat dormancy duration candidate gene TaCNGC-2A, using the candidate gene to obtain wheat plant panicle germination resistance duration judgment function markers, CAPS marker CNG2A, located in the TaCNGC-2A gene promoter- There is an A / T base variation at position 313, and its marker sequence is shown in SEQ ID NO.2. The corresponding marker primers were designed according to the marker sequence, and the length of the amplified product was 813bp. Amplified products were subjected to FokI restriction endonuclease (GGATGN 9 / ) It can be directly detected in 1.5% agarose gel electrophoresis after digestion at 37° C. for 3 hours. Among them, the CNG2A‑a allelic variation cannot be cleaved, while the CNG2A‑b allelic variation can be cleaved. The enzyme cleavage products are 584bp and 229bp respectively, and the plants carrying the CNG2A‑b allelic variation are more than those carrying CNG2A‑ Type a has a longer duration of ear germination resistance, and the marker provided by the invention can be directly applied to molecular marker-assisted wheat ear germination resistance breeding selection, and also helps to speed up the breeding process of ear germination-resistant varieties.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a wheat seed dormancy duration gene TaCNGC-2A and a functional marker thereof. Background technique [0002] About 35-40% of the world's population uses wheat as a staple food, and China is the world's largest wheat producer and consumer. Therefore, high yield, stable yield and high quality have always been important goals of wheat breeding (He Zhonghu et al. 2018). However, if the wheat harvest period encounters rainy weather, it is easy to germinate on the ear (abbreviated as ear germination). If the rainy weather lasts for a long time, it will intensify the ear germination and seriously affect the yield and quality. [0003] Seed dormancy is the main genetic factor of ear germination resistance, which includes two aspects of dormancy depth (level) and duration. Seed dormancy degree deep (or high level) and long duration have stronger resistance to ear germination. Howe...

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Problems solved by technology

However, previous studies mostly focused on the effect of seed dormancy depth on ear germination resistance at a certain period after harvest, ignoring the long duration of seed dormancy in the environment of longer duration of rainy wheat harvest season. The role of sex

Therefore, there are many QTL / gene reports on the depth of seed dormancy, such as the cloned TaSdr (2AS / 2BS, Zhang et al. 2014, 2017), TaVp-1 (3AL / 3BL, Yang et al. 2007; Chang et al. 2011), TaMFT and TaPHS1 (3AS, Nakamura et al. 2011; Liu et al. 2013), PM19-A1 (4AL, Barrero et al. 2015) and TaMKK3-A (4AL, Torada et al. 2016); there are few reports on the related QTL / genes regulating the duration of seed dormancy, To a certain extent, it hinders the cultivation of varieties with longer duration of panicle germination resistance

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