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Application of Tenovin-1 in preparing drug for preventing and controlling human herpes virus infection

A technology of tenovin-1, 1.tenovin-1 is applied in the directions of antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effect of inhibiting the production of infectious herpes virus particles

Inactive Publication Date: 2018-10-09
武汉威立得生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of Tenovin-1 in preparing drug for preventing and controlling human herpes virus infection
  • Application of Tenovin-1 in preparing drug for preventing and controlling human herpes virus infection
  • Application of Tenovin-1 in preparing drug for preventing and controlling human herpes virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Activity detection of Tenovin-1 against KSHV lysis phase replication

[0042] 1. Experimental materials

[0043] 1.1 Cells, viruses

[0044] iSLK.219 cells were donated by Prof. Dr. Don Ganem. The cells are SLK cells containing recombinant virus rKSHV.219 and doxycycline (Doxycycline) regulated RTA expression system. In this cell, the RTA expression sequence was constructed into the pRetro-X Tet-ON inducible expression system (Clontech, Mountainview, CA), the expression of which was regulated by doxycycline. In the presence of doxycycline, RT A expression can occur; 293T cells are preserved in our laboratory and purchased from the American Type Culture Collection (ATCC).

[0045] Tenovin-1 was purchased from Biochempartner.

[0046] 1.2 Reagents

[0047] DMEM medium, 1640 medium and FBS were purchased from GIBCO company; Alamarblue activity detection kit was purchased from Promega company; SYBR mixture (iTaq TM Universal Green Supermix) was purchased fr...

Embodiment 2

[0067] Embodiment 2: Detection of Tenovin-1 anti-HSV-2 activity

[0068] 1. Experimental materials

[0069] 1.1 Cells, viruses

[0070] Preserved by Vero laboratory and purchased from American Type Culture Collection (ATCC). HSV-2 is the G strain and is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0071] 1.2 Reagents

[0072] DMEM medium and FBS were purchased from GIBCO company; Alamarblue activity detection kit was purchased from Promega company; crystal violet staining solution.

[0073] 1.3 Experimental instruments

[0074] The multi-labeled microplate reader and the high-content cell analyzer were purchased from PerkinElmer Company; the cell incubator was a product of Thermo Company.

[0075] 2. Experimental methods and results

[0076] 2.1 Cytotoxicity detection of Tenovin-1 on Vero cells.

[0077] (1) Vero cells were divided into 8 × 10 3 Cells / well were seeded in 96-well cell culture plates in DMEM medium supplemented with 10% fe...

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PUM

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Abstract

The invention provides a use of Tenovin-1 in preparing a drug. The drug is used for treating or preventing herpes virus infection. According to the embodiment of the invention, the Tenovin-1 can be effectively used for treating or preventing herpes virus infection. Detection on herpes viruses in different subtypes shows that, the Tenovin-1 has the activity of broad-spectrum anti-herpes viruses, ishigh in selection index, can be developed into a drug for treating herpes virus infection diseases and has a wide application prospect.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular, the present invention relates to the application of Tenovin-1 or a pharmaceutically acceptable salt thereof in the preparation of a medicine for treating or preventing herpes virus infection. Background technique [0002] Herpesvirus is a kind of enveloped virus with double-stranded DNA genome, which can infect animals and humans widely. Epidemiological surveys show that 90% of adults carry one or more herpes viruses. Therefore, herpes virus infection is an important restrictive factor that endangers people's healthy life and life and property. At present, there are eight kinds of herpes viruses that can infect people, mainly including: Herpes simplex virus-1 (Herpes simplex virus-1, HSV-1), herpes simplex virus type II (Herpes simplex virus-2, HSV-2), Varicella-zoster virus (Varicellazoster virus, VZV), Epstein-Barr virus (EBV), cytomegalovirus (Cytomegalovirus, CMV), human he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/17A61K45/06A61P31/22
CPCA61K31/17A61K45/06A61P31/22A61K2300/00
Inventor 陈绪林陈军刚刘春兰其他发明人请求不公开姓名
Owner 武汉威立得生物医药有限公司
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