Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanofiber membrane loaded with prebiotics and/or probiotics as well as preparation and application

A nanofiber membrane and probiotic technology, applied in textiles, papermaking, non-woven fabrics, etc., can solve problems such as affecting the activity of probiotics, and achieve the effects of high porosity, expanding application space and simple process

Active Publication Date: 2018-09-28
SOUTH CHINA UNIV OF TECH
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, probiotics can only exert their beneficial function when ingested in a certain amount, and the food processing, storage and digestion environment will affect the activity of probiotics
However, there is no systematic report on prebiotic electrospinning

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanofiber membrane loaded with prebiotics and/or probiotics as well as preparation and application
  • Nanofiber membrane loaded with prebiotics and/or probiotics as well as preparation and application
  • Nanofiber membrane loaded with prebiotics and/or probiotics as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Probiotic effect test of different prebiotics on probiotics:

[0036] (1) Select four kinds of Lactobacillus (Lactobacillus casei, L.casei; Lactobacillus paracasei, L.paracasei; Lactobacillus plantarum, L.plantarum; Lactobacillus acidophilus, L.acidophilus) in the MRS medium, and Two kinds of bifidobacteria (B.adolescence; B.bifidum; B.bifidum) in the modified MRS medium (peptone 25g, beef extract 25g, yeast extract 12.5g, glucose 20g, sodium acetate 12.5 g, dipotassium hydrogen phosphate 5g, ammonium citrate 5g, magnesium sulfate 0.5g, manganese sulfate 0.13g, L-cysteine ​​1.25g, Tween-80 2.5g, 1000mL water, pH 7.0) at 37℃ Cultivate under oxygen condition for 24h, then transfer and continue to activate for 18h, and obtain a concentration of 10 9 CFU / mL of bacterial liquid.

[0037] (2) Select three kinds of oligosaccharides (fructo-oligosaccharide FOS, galacto-oligosaccharide GOS and isomalt-oligosaccharide IMO) prebiotics to replace the glucose in the MRS medium and...

Embodiment 2

[0045] The probiotic effect test of different concentrations of fructo-oligosaccharides (FOS) on probiotics:

[0046] The difference between this embodiment and Example 1 is that fructooligosaccharides of different concentrations (0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, w / v) are selected to replace the fructooligosaccharides in the MRS and improved MRS medium Glucose (carbon source), the absorbance value (OD 600 ) and pH. Comparison of the probiotic effects of different concentrations of fructooligosaccharides on four types of Lactobacillus and two types of Bifidobacteria figure 2 shown. figure 2 The results showed that with the increase of fructo-oligosaccharide addition, its prebiotic effect on probiotics showed a trend of first strengthening and then stabilizing.

Embodiment 3

[0048] The preparation of a nanofibrous membrane loaded with FOS of the present embodiment, the specific steps are as follows:

[0049] (1) Dissolve polyvinyl alcohol in sterile deionized water and stir at 80° C. for 1 h to prepare a 6 wt.% polyvinyl alcohol solution, and cool to room temperature. Then add 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% (w / w) fructo-oligosaccharides and mix uniformly to obtain polyvinyl alcohol / fructo-oligosaccharides spinning solution with different fructo-oligosaccharide concentrations .

[0050] (2) Inhale the resulting polyvinyl alcohol / fructooligosaccharide spinning solution into a 5mL sterile syringe, use a 0.6mm needle, adjust the distance from the needle to the receiver to 14cm, adjust the voltage to 16kV, and the flow rate to 0.5mL / h, the temperature is 25° C., the humidity is 40%, spinning for 1 h, and FOS-loaded nanofiber membranes (PVA / FOS) with different concentrations of fructo-oligosaccharides are obtained respectively.

[0051] The visco...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of functional food and discloses a nanofiber membrane loaded with prebiotics and / or probiotics as well as preparation and an application. Polyvinyl alcoholis dissolved in sterile deionized water, and a polyvinyl alcohol spinning solution is obtained; prebiotics are added to the polyvinyl alcohol spinning solution, uniform stirring is performed, and a polyvinyl alcohol / prebiotic spinning solution is obtained; a probiotic solution is added to the polyvinyl alcohol spinning solution or the polyvinyl alcohol / prebiotic spinning solution, uniform stirring is performed, and a polyvinyl alcohol / probiotic spinning solution or a polyvinyl alcohol / prebiotic / probiotic spinning solution is obtained; the nanofiber membrane loaded with prebiotics and / or probiotics is prepared from the spinning solution through electrospinning. The nanofiber membrane loaded with prebiotics and / or probiotics can improve the activity and thermal stability of probiotics to agreater extent.

Description

technical field [0001] The invention belongs to the technical field of functional food, and in particular relates to a nanofiber film loaded with prebiotics and / or probiotics, as well as its preparation and application. Background technique [0002] Probiotics, also known as live bacterial preparations, microecological regulators, ecological products, probiotics, etc., are a type of active microorganisms that can promote the ecological balance of the host's intestinal microbial flora and have beneficial effects on the host's health and / or physiological functions . However, probiotics can only exert their beneficial function when ingested in a certain amount, and the food processing, storage and digestion environment will affect the activity of probiotics. Therefore, it is urgent to develop a new technology to enable probiotics to survive not only under the conditions of production, processing and packaging, but also when passing through the gastrointestinal tract, so that p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/728D04H1/4309D04H1/413
CPCD04H1/413D04H1/4309D04H1/728
Inventor 吴虹冯坤宗敏华翟梦宇
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com