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A test strip that significantly improves lateral flow immunochromatography

An immunochromatographic detection and immunodetection technology, which is applied in measurement devices, analytical materials, instruments, etc., can solve the problems of low detection limit, short in vitro mixing time, and high detection sensitivity

Active Publication Date: 2021-09-24
上海艾瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, there is still a lack of an immunochromatographic test strip with high detection sensitivity, short in vitro mixing time, and low detection limit in this field.

Method used

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  • A test strip that significantly improves lateral flow immunochromatography
  • A test strip that significantly improves lateral flow immunochromatography
  • A test strip that significantly improves lateral flow immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Take the determination of troponin cTnI standard as an example.

[0088] Use anti-cTnI mouse monoclonal antibody to label fluorescent microspheres, use anti-cTnI goat polyclonal antibody as capture antibody to draw on the membrane as a detection line, and use goat anti-mouse polyclonal antibody as quality control line to draw membrane antibody. The cTnI standard substance and the fluorescent microspheres of the labeled antibody were mixed in vitro for 30 seconds, then dropped onto the sample pad, and then measured after lying flat for 900 seconds. The probe of the detector moves from the direction of the sample pad to the direction of the absorbent pad, and a total of 180 points including the T line and the C line are read.

[0089] Schematic such as Figure four , calculate the values ​​of TY and CY by computer, integrate the peak area of ​​T line and C line to get the values ​​of TA and CA, and use TA / TA+CA to calculate the value of TAP to calibrate the difference ca...

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Abstract

The invention provides a method for significantly improving the detection signal intensity of a lateral flow immunochromatography test strip, and the method for improving the detection signal intensity includes the steps: (a) adding the sample and the antibody labeled on the fluorescent microspheres to the (b) the analyte in the sample combines with the antibody labeled on the fluorescent microsphere to form a complex to be tested, and the complex to be tested gradually diffuses to the nitrocellulose membrane along with the chromatographic flow; (c) Divide the detection line and the quality control line on the nitrocellulose membrane; (d) carry out modification by punching holes on the nitrocellulose membrane between the sample pad and the detection line, and obtain the modification formed by 1-10 rows of modified holes Area. The present invention can achieve the purpose of increasing the probability of the compound to be detected being captured, reducing the mixing time in vitro, improving the test sensitivity, reducing the measurement limit value, and / or improving the detection signal.

Description

technical field [0001] The invention relates to an immunochromatography test strip, in particular to a lateral flow immunochromatography test strip which can significantly improve the lateral flow immunochromatography. Background technique [0002] Lateral flow chromatography has the advantages of low cost, simple operation, quickness, and portability, and is an important part of the field of medical diagnosis. [0003] At present, the common lateral flow immunochromatography method on the market is mainly the double-antibody sandwich method, also known as the sandwich detection method, which consists of a polyvinyl chloride bottom plate, a nitrocellulose membrane, a sample pad, and an absorbent pad. Generally, in lateral flow immunofluorescence chromatography, human or animal body fluid samples are mixed with fluorescent particle-labeled antibodies in vitro to form antigen-antibody complexes, which are added to the sample pad, and the complexes diffuse with the chromatograp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 李秋萍王佩瑞叶一肖琨倪晓涛陆亮朱轩仪杨茜茹操凤文李欢
Owner 上海艾瑞德生物科技有限公司
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