Auxiliary safe docking device of human embryo freezing carrier poles under liquid nitrogen and using method of auxiliary safe docking device

Abstract

The invention discloses an auxiliary safe docking device of human embryo freezing carrier poles under liquid nitrogen and a using method of the auxiliary safe docking device. According to the auxiliary safe docking device of the human embryo freezing carrier poles under the liquid nitrogen and the using method of the auxiliary safe docking device, disclosed by the invention, limiting and fixing are carried out by nesting protection caps of freezing carrier poles in cylindrical holes, meanwhile, the freezing carrier poles are arranged in cross grooves and are in face-to-face docking, the wholedocking process can be completed in the cross grooves formed in preset paths, docking observation under the liquid nitrogen requires not to be carried out by naked eyes, one-time success can be ensured, potential damage of a traditional method to embryos or staff can be effectively avoided, the operation is simple and convenient, and an effect is reliable.

Description

technical field [0001] The invention relates to a medical appliance, in particular to an auxiliary safety docking device for human embryo freezing rods under liquid nitrogen and a use method thereof. Background technique [0002] With the liberalization of the country's comprehensive "two-child" policy, more and more women have reproductive needs. However, due to the superimposed influence of factors such as increasing age, environmental pollution, high work and life pressure, and unhealthy lifestyles, about 1 out of every 7 couples in my country cannot carry out normal fertility, so they have to resort to assisted reproduction (test-tube baby). technology. [0003] However, when performing IVF-ET (in vitro fertilization-embryo transfer) operations in the embryonic laboratory, it often happens that the number of available embryos is greater than the number of transplanted embryos or the endometrium of the woman is not in the best embryo transfer period. Cryopreservation of ...

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Problems solved by technology

However, since the entire docking process must be observed with the naked eye, and in order to minimize the potential damage of light to embryos in the embryonic laboratory, the entire operating environment is relatively dark. In addition, liquid nitrogen is volatile, and the nitrogen vapor above the liquid nitrogen will hinder it. In line of sight observation, light will be refracted between liquid nitrogen and air, making it difficult to operate under naked eye observation

It is impossible for the experimenters to connect the frozen loading rod and the protective cap cover too deep under the liquid nitrogen, because the deeper the loading rod is, the weaker the light reflected to the eyes is, and the more difficult it is to observe; The protective cap cover is immersed in liquid nitrogen too shallow, because once the forceps are not in place, the end of the rod carrying the embryos will float out of the liquid surface and cause fatal damage to the embryos

Even if the loading rod and the protective cap are in the proper position under the liquid nitrogen, due to the above-mentioned factors such as dim light, liquid nitrogen volatilization, interface refraction, etc., the docking process often needs to be completed many times, and this exists in the Risk of inadvertent contact of the protective cap with the embryo during repeated docking procedures

In addition, in order to ensure the docking effect and better see the docking position, the experimenters put their heads as close to the liquid nitrogen liquid surface as possible to observe, and at the same time, the experimenters also inhaled more volatilized liquid nitrogen. damage to the body

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