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Gene detection kit and gene detection method for predicating risk of mineral deficiency in human bodies

A gene detection and mineral technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of harsh storage conditions, low accuracy, and painful sample collection process, and achieve low sampling conditions and guarantee High precision and simple storage conditions

Inactive Publication Date: 2018-09-04
GUANGZHOU HOMEY HEALTH TECH
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AI Technical Summary

Problems solved by technology

[0005] The main problems of existing detection methods for minerals in the human body are: high requirements for collection and sampling conditions, harsh storage conditions, painful sample collection process, long detection process, poor reliability and low accuracy of detection results

Method used

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  • Gene detection kit and gene detection method for predicating risk of mineral deficiency in human bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0113] 1. Extraction of sample DNA:

[0114] Sampling: Use a cotton swab to wipe the inside of the cheek 10 times. Do not eat or drink for 30 minutes before sampling.

[0115] deal with:

[0116] 1. Transfer the swab into a 2mL centrifuge tube, cut off the swab part from its stem with scissors, and add 400μL of solution A.

[0117] 2. Add 20 μL of RNaseA (10 mg / mL) to the solution, and place it at 55° C. for 15 minutes.

[0118] 3. Add 20 μL of proteinase K (10 mg / mL), mix thoroughly by inversion, and digest in a 55°C water bath for 60 minutes. During the digestion period, it is recommended to invert the centrifuge tube and mix once every ten minutes.

[0119] 4. Add solution B in a volume of 400 μL, and mix thoroughly by inversion. If a white precipitate appears, it can be placed at 75°C for 15-30 minutes, and the precipitate will disappear without affecting subsequent experiments.

[0120] 5. Transfer the solution to a new centrifuge tube, add 400 μL of absolute ethanol,...

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Abstract

The invention provides a gene detection kit and a gene detection method for predicating the risk of mineral deficiency in human bodies. The kit comprises one or more of a first reagent for detecting MT1A gene mutation sites rs11640851, a second reagent for detecting SLC30A8 gene mutation sites rs13266634, and a third reagent for detecting SLC30A3 gene mutation sites rs11126936. The gene detectionkit and the gene detection method have the advantages that real-time sampling and detecting for the risk of the mineral deficiency in the human bodies are realized, requirements of collecting and sampling conditions are low, storage conditions are simple, pain is avoided during sampling, and the detection process is short in time-consuming; the risk of the mineral deficiency in the human bodies ispredicated according to the gene detection method, so that predication accuracy is improved greatly, high detection accuracy is guaranteed, and influences of the joint effect of genetic factors and other factors on the risk of the mineral deficiency in the human bodies are evaluated effectively.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a genetic detection kit and detection method for predicting human mineral deficiency risk. Background technique [0002] Minerals are compounds or natural elements that occur naturally in the Earth's crust. Zinc is an important component of minerals. It participates in many important life processes. Zinc is a component of more than 200 enzymes and DNA and RNA activities in the body, and is an essential substance for growth and development. It can regulate the secretion of hormones from organs such as testes and ovaries, help effectively relieve stress, and promote the health of the nervous system and brain, as well as oral mucosal repair and wound healing. In addition, it is also indispensable for growing children and fetuses, and plays a very important role in the growth and development of the human body, the formation of bones and teeth, the growth of hair, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/156
Inventor 陈希阳黄智敏
Owner GUANGZHOU HOMEY HEALTH TECH
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