Liquid drop cultivation method of three-dimensional organs

Abstract

The invention relates to the fields of a biological technology and a cell culture technology, in particular to a liquid drop cultivation method of three-dimensional organs and application of the three-dimensional organs to biomedicine. A pipette tip and the conventional cell culture method are combined, a liquid drop with spherical conformation is formed by utilizing the pipette tip to serve as aculture carrier, and a millimeter-sized organ tissue is prepared by performing cultivation in the liquid drop. The ideal organ-like liquid drop culture condition is obtained by screening and analyzingfactors such as the cut position of the pipette tip, the volume of the injected liquid as well as the angle of the contact angle between the liquid drop and the section. Meanwhile, the form (shape and size) of the three-dimensional organ is controlled flexibly and effectively by adjusting the condition in a timely manner. In addition, the method provided by the invention has the advantages of being simple and convenient to operate, low in culture cost, high in culture efficiency and the like. The millimeter-sized three-dimensional organ tissue prepared according to the cultivation method canmeet the requirement of performing in-vitro medicine screening in the early personalized treatment stage.

Description

technical field [0001] The invention relates to the fields of biotechnology and cell culture technology, in particular to a three-dimensional organoid droplet culture method and its application in biomedicine. Background technique [0002] Organoids are a class of three-dimensional cell cultures that contain some of the key properties of their representative organs. This type of in vitro culture system includes a population of self-renewing stem cells that can differentiate into organ-specific cell types that are compatible with the corresponding organ. Similar spatial organization can reproduce part of the functions of the corresponding organs. [0003] In recent years, biomedical research based on 3D organoid models has made great progress. Because organoids have the ability of self-renewal, self-organization, and can display organ functions to a considerable extent, they can not only serve as a substrate to provide physical support, but also provide biochemical cues, reg...

AI Technical Summary

Problems solved by technology

[0007] The present invention combines the pipette tip (or pipette) with the conventional cell culture method, and utilizes the droplet of the spherical conformation formed by the pipette tip as the culture carrier, in which the millimeter-scale three-dimensional organoid tissue is cultured and prepared. The method of the present invention passes Controlling the size and shape of the droplets can produce a unique ability to control the shape and structure of the prepared three-dimensional organoids, thereby overcoming the inability of the existing three-dimensional organoid culture technology to effectively control the organ morphology (shape and size) during the culture process defect

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