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Pseudomonas aeruginosa detection membrane and preparation method thereof and method for detecting bacteria

A Pseudomonas aeruginosa, detection layer technology, applied in the direction of measuring devices, instruments, analysis materials, etc., can solve the problem of high machine cost

Inactive Publication Date: 2018-08-24
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These advanced technology inspection methods require professional and technical personnel to operate, and the cost of the machine is relatively high

Method used

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  • Pseudomonas aeruginosa detection membrane and preparation method thereof and method for detecting bacteria
  • Pseudomonas aeruginosa detection membrane and preparation method thereof and method for detecting bacteria
  • Pseudomonas aeruginosa detection membrane and preparation method thereof and method for detecting bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;

[0045] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 1 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;

[0046] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofibrous membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-based fluorescent molecules at a concentration of 3 g / L for 20 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 3g / L for 20 minutes, then taken out and washed with clean water, ...

Embodiment 2

[0048] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;

[0049] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 0.5 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;

[0050] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofiber membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-like fluorescent molecules at a concentration of 1 g / L for 30 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 2g / L for 30 minutes, then taken out and washed with clean water, a...

Embodiment 3

[0052] 1) Preparation of nanofiber film: uniformly spray polyethylene-polyvinyl alcohol nanofiber suspension on the surface of ordinary non-woven fabrics, dry after film formation, and separate to prepare polyvinyl alcohol- Polyethylene nanofibrous membrane;

[0053] 2) Prepare a surface-activated nanofiber membrane: immerse the nanofiber membrane prepared in step 1) in a sodium hydroxide solution with a concentration of 2.5 mol / L for about 30 minutes to obtain a surface-activated nanofiber membrane;

[0054] 3) Preparation of bacteria detection membrane: immerse the surface-activated nanofiber membrane prepared in step 2) in an aqueous solution of glycosyl polymer functionalized with fluorescein-based fluorescent molecules at a concentration of 4 g / L for 20 minutes, and then take out After being washed with clean water, it was immersed in an aqueous solution of phenylboronic acid polymer with a concentration of 4g / L for 20 minutes, then taken out and washed with clean water, ...

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Abstract

The invention relates to a Pseudomonas aeruginosa detection membrane and a preparation method thereof and a method for detecting bacteria, and belongs to the technical field of biological detection. The Pseudomonas aeruginosa detection membrane comprises a substrate layer, and comprises a detection layer, the detection layer is disposed on the substrate layer, the substrate layer comprises a nanofiber membrane, the detection layer comprises a fluoroboron fluorescent molecule functionalized glycopolymer and a phenylboronic acid polymer, wherein the fluoroboron fluorescent molecule functionalized glycopolymer and the phenylboronic acid polymer are uniformly and alternately assembled on the surface of the nanofiber membrane by layer-by-layer self-assembly. The detection membrane is not only low in cost but also capable of quantitatively detecting Pseudomonas aeruginosa.

Description

technical field [0001] The invention relates to the detection of Pseudomonas aeruginosa, belongs to the technical field of biological detection, and in particular relates to a Pseudomonas aeruginosa detection membrane and a preparation method thereof and a method for detecting bacteria thereof. Background technique [0002] Pseudomonas aeruginosa, formerly known as Pseudomonas aeruginosa, is widely distributed in nature and is a common conditional pathogen. It can survive for a long time in a humid environment and has strong resistance to drying. Under other inducing conditions such as reduced body resistance or disease, it is easy to cause infection and disease, and the harm is wide. If unfortunately infected with this bacteria, it will generally cause abscess and sepsis, and can cause death in severe cases. [0003] In addition to causing contamination in hospital settings, P. aeruginosa is also commonly found in contaminated water, and may be present even in water source...

Claims

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Application Information

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IPC IPC(8): G01N21/64C08J7/12C08J7/00C08J5/18C08L23/06C08L29/04
CPCG01N21/6428C08J5/18C08J7/00C08J7/12C08J2323/06C08J2329/04C08J2423/06C08J2429/04G01N21/6486G01N2021/6432
Inventor 鲁振坦王栋余振国张佳琪王跃丹
Owner WUHAN TEXTILE UNIV
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