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Dragon tree total flavone extraction method and application of total flavone

An extraction method and a technology of total flavonoids, which are applied in the field of extraction of total flavonoids from Dracaena dracaena, to achieve obvious effects of inhibiting the proliferation of gastric cancer cells, high yield, simple and feasible extraction method

Inactive Publication Date: 2018-08-24
中国医学科学院药用植物研究所云南分所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the present invention finds that the total flavonoids extracted from Dracaena have more significant anti-tumor activity, especially have a good inhibitory effect on gastric adenocarcinoma, and no research has been reported so far.

Method used

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  • Dragon tree total flavone extraction method and application of total flavone
  • Dragon tree total flavone extraction method and application of total flavone
  • Dragon tree total flavone extraction method and application of total flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 100 g of the resinous Dracaena medicinal material, add 10 times the amount of ethyl acetate to reflux for extraction for 2 hours, filter, collect the filtrate, concentrate under reduced pressure to obtain 10.5 g of extract with a density of 1.05. The obtained extract was added into a 150 g macroporous resin packed column, eluted with 200 ml of 85% ethanol, and the eluate was collected to obtain the total flavonoids of Dracaena dracaena. The total flavonoids were determined by ultraviolet spectrophotometer with dragon blood B as the standard, and the total flavonoids content was 58.63%.

[0020] Take 200g of resin-containing Dracaena medicinal material, add 5 times the amount of n-butanol to reflux for extraction for 2 hours, filter, collect the filtrate, and concentrate under reduced pressure to obtain 25g of extract with a density of 1.10. The obtained extract was added into a 300 g macroporous resin packed column, eluted with 500 ml of 90% ethanol, and the eluate...

Embodiment 2

[0022] Taking Dracaena anti-gastric adenocarcinoma effective part extract (Example 1 extract) as test drug and gastric adenocarcinoma cell line AGS as object, measure the IC of AGS 50 . The method is as follows: take AGS cells in logarithmic phase, dilute the cell concentration with DMEM complete medium containing 10% calf fetal bovine serum, 1×10 per well 5 200 μL of each cell was seeded in a 96-well plate at 37°C, 5% CO 2 Adhesive culture in the incubator for 24 hours. After aspirating the medium, add 100 μL of serum-free drug-containing medium to each well, so that the final concentrations of total flavonoids in blood trees are 100 μg / mL, 50 μg / mL, 25 μg / mL, 12.5 μg / mL, 6.3 μg / mL and 3.1 μg / mL, 3 replicate wells per mass concentration, the blank control group was added with the same amount of serum-free medium, and the excipient control group (serum-free medium containing 0.5% DMSO) was set at the same time, at 37 ° C, 5% CO 2 Cultivate in the incubator for 24h. Take o...

Embodiment 3

[0025] Example 3 Dracaena extract induces inhibition of proliferation of gastric adenocarcinoma cells

[0026] Dilute the AGS cell concentration with DMEM complete medium containing 10% calf fetal bovine serum, 1×10 per well 5 cells / ml cell suspension was seeded in a 96-well plate at 37°C, 5% CO 2 Adhesive culture in the incubator for 24 hours. After aspirating the medium, add 100 μL of serum-free drug-containing medium to each well, so that the final concentrations of total flavonoids of Dracaena 28 μg / mL, 14 μg / mL, 7 μg / mL, and 0 μg / mL were treated for 48 hours and 28 μg / mL respectively. 0h, 12h, 24h, 48h. There were 3 replicate wells per mass concentration, the same amount of serum-free medium was added to the blank control group, and the excipient control group (serum-free medium containing 0.5% DMSO) was set at the same time, at 37°C, 5% CO 2 in the incubator. The cells of each concentration were taken out according to different culture time and observed and photograp...

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Abstract

The invention discloses a dragon tree total flavone extraction method and an application of the total flavone. The extraction method includes the steps: extracting resiniferous dragon tree medicinal materials by organic solvents; enriching macroporous resin to obtain dragon tree total flavone. The organic solvents are ethyl acetate or n-butyl alcohol. The dragon tree total flavone has an obvious effect on inhibition of gastric adenocarcinoma cell proliferation and can be applied to treatment of gastric adenocarcinoma. The extraction method is simple, feasible and high in yield, and the obtained dragon tree total flavone has the obvious effect on inhibition of gastric adenocarcinoma cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a method for extracting total flavonoids from Dracaena dracaena and its application. Background technique [0002] Gastric adenocarcinoma is a type of gastric cancer, which is caused by malignant transformation of gastric gland cells, so it is called adenocarcinoma. The incidence of gastric adenocarcinoma accounts for 95% of gastric malignant tumors, relatively rare are lymphoma (only in the stomach) and leiomyosarcoma. The incidence of gastric cancer varies around the world; for example, Japan, Chile, and Iceland have very high rates. In the United States, gastric cancer is most common among northerners, the poor, and blacks, but its incidence has decreased to about 8.1 million, and it is the seventh leading cause of cancer death. However, in Japan, the incidence of gastric cancer has decreased, but is still It is the most common malignant tumor, and its ...

Claims

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Application Information

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IPC IPC(8): A61K36/88A61P35/00
CPCA61K36/88A61K2236/35A61K2236/51A61K2236/55A61P35/00
Inventor 李光吕亚娜李宜航罗云马国需李学兰陈曦陈德力
Owner 中国医学科学院药用植物研究所云南分所
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