Extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of active components in agriculture
An extraction method and technology of active ingredients are applied in the field of botanical pesticides to achieve the effect of inhibiting proliferation
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Embodiment 1
[0037] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with ethanol at a material-to-liquid ratio of 1kg:3L (m / V), and subjected to four-stage microwave extraction to obtain the active ingredients of the king jujube. The specific process As shown in Table 1:
[0038] Table 1. The extraction process of the ethanol extract of Wang Zaozi
[0039]
[0040] The active ingredients of Wangzaozi obtained in the examples were determined by HPLC, and the active ingredient content is shown in Table 2:
[0041] Table 2. Effective ingredients and content of ethanol extract of Wangzaozi
[0042]
[0043]
[0044] Inoculate Pyricularia grisea and Pyricularia oryzae on PDA solid medium, culture for 5 days in the dark at a constant temperature of 25℃, after the hyphae grow evenly, punch the PDA medium filled with hypha by a 0.5cm puncher, and inoculate them respectively On the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25...
Embodiment 2
[0049] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with acetone at a material-to-liquid ratio of 1kg:3L (m / V), and subjected to four-stage microwave extraction. The specific process is shown in Table 4:
[0050] Table 4. The extraction process of acetone extract of Wangzaozi
[0051]
[0052] Pear anthracnose, penicillium, black spot pathogen and rice blast fungus were inoculated on PDA solid medium and cultured in the dark at 25℃ for 5 days. After the hyphae grew evenly, the 0.5cm punch was punched to cover the hyphae The PDA medium was inoculated on the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25, 0.1, 0.05 mg / mL of the extract of Example 2 respectively. Incubate at 25°C for 7 days and record the inhibition zone. The inhibition rate is shown in Table 5:
[0053] Table 5. Antibacterial rate of acetone extract of Wangzaozi
[0054]
[0055] It can be seen that the acetone extract of Wangzaozi has...
Embodiment 3
[0057] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with chloroform at a material-to-liquid ratio of 1kg:3L (m / V), and then subjected to four-stage microwave extraction. The specific process is shown in Table 6:
[0058] Table 6. Extraction process of chloroform extract of Wang Zaozi
[0059]
[0060] Inoculate Aspergillus niger, Pear rotifer, Pear anthracnose, Penicillium, Pear black spot and Pyricularia oryzae on PDA solid medium, culture for 5 days in the dark at a constant temperature of 25°C, and 0.5 cm after the hyphae grows evenly. The PDA medium filled with hyphae was perforated by the hole tool and inoculated on the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25, 0.1, 0.05 mg / mL of the extract of Example 3, respectively. Incubate at 25°C for 7 days and record the inhibition zone. The inhibition rate is shown in Table 7:
[0061] Table 7. Antibacterial rate of chloroform extract of Wang Zaozi
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