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Formaldehyde-free specimen preservation liquor and preparation method thereof

A technology for preserving liquids and specimens, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of yellowing, specimen hardening, mildew, etc., and achieve the effects of slow volatilization, natural color, and elimination of damage.

Inactive Publication Date: 2018-08-24
黄名义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the specimens preserved in formaldehyde solution for a long time will have mildew, yellowing, and hardening of the specimens.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A formaldehyde-free specimen preservation solution, which is prepared by mixing the following raw materials in proportions by weight:

[0023] 10 parts of methanol, 5 parts of phenoxyethanol, 3 parts of potassium sorbate, 7 parts of glycerin, 2 parts of potassium nitrate, 1 part of benzoic acid, 0.5 parts of picric acid, 1 part of isothiazolone, 0.4 parts of thymosic acid, 0.9 parts of sodium chloride 2 parts, 2 parts of disodium hydrogen phosphate, 0.8 parts of penicillin, 0.8 parts of streptomycin, and 0.5 parts of essence.

[0024] The preparation method of the above-mentioned formaldehyde-free specimen preservation solution comprises the steps:

[0025] (1) Put potassium sorbate, potassium nitrate, benzoic acid, picric acid, isothiazolone, thymelic acid, sodium chloride, disodium hydrogen phosphate, penicillin, streptomycin, essence into methanol and stir evenly, set aside;

[0026] (2) After the product in step (1) is completely dissolved, add phenoxyethanol and g...

Embodiment 2

[0031] A formaldehyde-free specimen preservation solution, which is prepared by mixing the following raw materials in proportions by weight:

[0032] 15 parts of methanol, 6 parts of phenoxyethanol, 4 parts of potassium sorbate, 9 parts of glycerin, 3 parts of potassium nitrate, 1.5 parts of benzoic acid, 0.6 parts of picric acid, 1.5 parts of isothiazolone, 0.5 parts of thymosic acid, 1.2 parts of sodium chloride 1 part, 3 parts of disodium hydrogen phosphate, 1 part of penicillin, 1 part of streptomycin, and 1 part of essence.

[0033] The preparation method of the above-mentioned formaldehyde-free specimen preservation solution comprises the steps:

[0034] (1) Put potassium sorbate, potassium nitrate, benzoic acid, picric acid, isothiazolone, thymelic acid, sodium chloride, disodium hydrogen phosphate, penicillin, streptomycin, essence into methanol and stir evenly, set aside;

[0035] (2) After the product in step (1) is completely dissolved, add phenoxyethanol and glyce...

Embodiment 3

[0040] A formaldehyde-free specimen preservation solution, which is prepared by mixing the following raw materials in proportions by weight:

[0041] 20 parts of methanol, 7 parts of phenoxyethanol, 5 parts of potassium sorbate, 11 parts of glycerin, 4 parts of potassium nitrate, 2 parts of benzoic acid, 0.7 parts of picric acid, 2 parts of isothiazolone, 0.6 parts of thymosic acid, 1.5 parts of sodium chloride 4 parts, 4 parts of disodium hydrogen phosphate, 1.2 parts of penicillin, 1.2 parts of streptomycin, 1.5 parts of essence.

[0042] The preparation method of the above-mentioned formaldehyde-free specimen preservation solution comprises the steps:

[0043] (1) Put potassium sorbate, potassium nitrate, benzoic acid, picric acid, isothiazolone, thymelic acid, sodium chloride, disodium hydrogen phosphate, penicillin, streptomycin, essence into methanol and stir evenly, set aside;

[0044] (2) After the product in step (1) is completely dissolved, add phenoxyethanol and gl...

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PUM

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Abstract

The invention discloses a formaldehyde-free specimen preservation liquor. The preservation liquor is prepared from the following raw materials in parts by weight: 10-20 parts of methanol, 5-7 parts ofphenoxyethanol, 3-5 parts of potassium sorbate, 7-11 parts of glycerin, 2-4 parts of potassium nitrate, 1-2 parts of benzoic acid, 0.5-0.7 parts of picric acid, 1-2 parts of isothiazolone, 0.4-0.6 parts of thymotic acid, 0.9-1.5 parts of sodium chloride, 2-4 parts of disodium hydrogen phosphate, 0.8-1.2 parts of penicillin, 0.8-1.2 parts of streptomycin and 0.5-1.5 parts of flavor. The preservation liquor does not contain formaldehyde, volatilizes slowly, does not have pungent smell, comprises easily available raw materials, has low cost, high safety and good preservation effect, does not mildew when stored for a long time, always keeps a soft state, can be repeatedly used, is not easy to damage and does not contain toxic or harmful substances and is green and environmentally friendly; harm of formaldehyde to human bodies is eliminated, and the burden on the environment is reduced.

Description

technical field [0001] The invention relates to the technical field of specimen preservation, in particular to a formaldehyde-free specimen preservation solution and a preparation method thereof. Background technique [0002] The formalin specimen preservation solution has a good fixation effect, which is due to the large amount of formaldehyde combined with the functional groups of the specimen protein, and after long-term storage, it further polymerizes to form paraformaldehyde. This process makes it form a moderate cross-link between protein peptide chains, which can not only kill bacteria, but also maintain the tissue shape of the specimen. However, formaldehyde and benzene have been identified as carcinogens by the World Health Organization and are listed as highly toxic chemicals, which can strongly irritate the eyes, skin, and mucous membranes of the respiratory tract, etc., and eventually cause immune dysfunction, liver damage, and nervous central system. It will le...

Claims

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Application Information

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IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 黄名义
Owner 黄名义
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