Sudden acute fulminating infectious disease emergency detection reserve kit and test method

Abstract

The invention provides a sudden acute fulminating infectious disease emergency detection reserve kit, which comprises a specific primer and probe combination for fluorescent quantitative PCR determination of a group of specific pathogenic microorganisms, a base buffer solution, an enzyme reagent, and diluting water. Specifically, the primer and probe are a tube of dry powdery mixture. The kit andthe test method provided by the invention have the beneficial effects that: with the fluorescent quantitative (probe method) core reagent provided by the kit, and by utilizing the characteristics of good sensitivity, specificity and stability of the technology, rapid emergency checking of various sudden acute infectious disease pathogenic microorganism targets in necessity can be realized simply.

Description

technical field [0001] The invention relates to the field of detection of pathogenic microorganisms, and more specifically relates to an emergency detection reserve kit and a detection method for sudden acute severe infectious diseases. Background technique [0002] In recent years, with the global movement of people and logistics and climate change and other factors, pathogens of various infectious diseases infect people and Formed and spread, leading to frequent outbreaks of major epidemics, which brought great harm to human health, especially some new, sudden, acute and severe infectious diseases, such as plague, MERS coronavirus, highly pathogenic avian influenza virus, yellow Infectious diseases caused by fever virus, Ebola virus, etc. Compared with common infectious diseases such as hepatitis, AIDS, and syphilis, the outbreak of such infectious diseases usually has the characteristics of sudden occurrence, overseas importation, and high social concern. It is very impo...

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Problems solved by technology

However, these methods are currently limited by primers, reaction conditions, and stability. Generally, the types of pathogens covered are about 5, and the stability, specificity, and sensitivity of multiple detection methods are complicated to optimize. Therefore, there is no such method on the market. Form a pathogen detection reserve kit with full coverage of known new, sudden and severe pathogens. In addition, the storage period is short, and the storage cost of front-line disease control or infectious disease prevention and control institutions is very high, and many new outbreaks occur Sometimes, it is often necessary to repurchase, it is difficult to achieve the "preparation" in the sense of full coverage target reserve, and it is often impossible to detect immediately when in use

In addition, the storage time of pathogen detection kits for a single target is short, generally 0.5-1 year, and the storage price of detection kits for multiple pathogens is also expensive, and the amplification and fluorescence signal acquisition conditions of different pathogen detection methods are different. The kit needs to be reconfigured in a targeted manner, and the inspection skills of the inspectors are also high, which limits the application of experimental detection technology in rapid on-site detection

Laboratories of national or provincial disease control or high-level infectious disease prevention and control institutions have self-developed and verified reserve fluorescent PCR methods, but such primers and probes are often synthesized based on mass numbers, and primers and probes of different lengths are used during work. The concentration must be diluted after complicated calculations. The complexity of this operation requires a high degree of specialization of the operators, and errors are prone to occur during multi-pathogen detection. At present, it can only be done in national or provincial institutions. It has the ability to detect pathogens with full coverage. Therefore, the corresponding epidemic detection time has to be delayed, which also greatly limits the promotion of emergency detection methods for emerging infectious diseases to front-line disease control or infectious disease diagnosis and treatment medical institutions.

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