Cell-metabonomics-based difference metabolite metabolic pathway of rubusoside lipotoxicity resistance and research method

Abstract

The invention discloses a cell-metabonomics-based difference metabolite metabolic pathway of rubusoside lipotoxicity resistance and a research method, relates to research methods of natural plant drugeffective component action mechanisms, and aims to solve the problem that an existing single pharmacology method cannot systematically and comprehensively evaluate the natural plant drug effective component action mechanisms. The method includes the steps of firstly, using ultrahigh performance liquid chromatography-tandem mass spectrum technology to detect and analyze palmitic acid induced rateinsulin oncocyte and rubusoside intervened intracellular metabolites; secondly, screening 12 potential lipotoxicity related difference metabolites, and identifying; thirdly, screening four metabolic pathways of the rubusoside lipotoxicity resistance and related enzymes and genes on the pathways, and analyzing. The method can comprehensively, efficiently and fast evaluate the rubusoside lipotoxicity resistance action mechanism from cell level in a bias-free manner, and a demonstrative basis is provided for natural plant drug effective component cell metabonomics and action mechanism illustration.

Description

technical field [0001] The invention belongs to the field of medicine. Specifically, it is a metabolic pathway and research method of differential metabolites of sweet tea against lipotoxicity based on cell metabolomics. Background technique [0002] Diabetes is a group of metabolic diseases characterized by high blood sugar, which has become the third major chronic disease that seriously endangers human health, of which type 2 diabetes accounts for more than 90%, and the cardiovascular and microvascular complications caused by it have become fatal and disabled. the main reason. Therefore, it is of great significance and urgency to find drugs that can prevent and intervene in the pathogenesis of type 2 diabetes and to clarify its mechanism of action for improving the health of our people. [0003] For a long time, medical researchers have been committed to the research on the pathogenesis and therapeutic drugs of type 2 diabetes. The pathogenesis of diabetes is complex, i...

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Problems solved by technology

[0007] The purpose of the present invention is to address the difficulty that the existing pharmacological methods cannot completely and comprehensively explain the mechanism of action of sweet tea in anti-lipotoxicity, and to provide a metabolic pathway and research on the differential metabolites of sweet tea in anti-lipotoxicity based on cell metabolomics method to provide a basis for the further development and utilization of sweet tea

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