Hyperspectral microscopic polarization imaging device and method

Abstract

The present invention discloses a hyperspectral microscopic polarization imaging device and method. The device comprises: a microscopic imaging device configured to perform amplification imaging observation of a sample and obtain images of the sample in different measurement conditions; a spectral filtering device configured to perform screening of optical wavelengths of an incident optical path and performing continuous single-band selection; a polarization modulation device configured to perform modulation of optical waves with sample polarization information; and an image processing unit configured to performing processing and calculation of light intensity images to obtain the single-band polarization parameter image of the sample. The method comprises the steps of: filtering a light source through a spectral filtering device, and allowing the light to be incident onto a sample; controlling a polarizing film to perform rotation for three times, and performing sampling of images; processing the collected images by the image processing unit, and obtaining a polarization data cube of the sample under a single wavelength; and screening out the next center wavelength by the spectralfiltering device, repeatedly performing rotation and image sampling of the polarizing film until sampling of each waveband is completed, and obtaining a sample polarization spectroscopic data set. The optical path is simple, the cost is low, and the measurement information is comprehensive.

Description

technical field [0001] The invention relates to the technical field of microspectral imaging, in particular to a hyperspectral microscopic polarization imaging device and method. Background technique [0002] Since the 1860s, spectroscopy has been used for qualitative and quantitative analysis of substances. After more than a hundred years of development, spectral analysis technology has gradually matured and been used in various fields. However, it was not until the 1980s that spectral technology was combined with two-dimensional imaging technology to produce spectral imaging technology, that is, map-spectrum integration. This technology is mainly used in the field of remote sensing, and is gradually expanding to fields such as agriculture, minerals, archaeology, and microscopic imaging. [0003] The existing micro-spectroscopy system is based on the principle of a push-broom imaging spectrometer, using a prism-grating-prism combination element to perform spectrum spectros...

AI Technical Summary

Problems solved by technology

However, the existing polarization technology uses full-band polarization, and does not consider the influence of different single-bands on polarization. The polarization characteristics will be affected by the wavelength, so that a polarization spectrum can be formed. Various information of the object is included in these polarization characteristics.

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