A method for constructing a spontaneous anemia mouse model with leukocyte and thrombocytosis
A technology of thrombocytosis and mouse models, applied in other methods of inserting foreign genetic materials, chemical instruments and methods, biochemical equipment and methods, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 Construction of spontaneous anemia model mice (i.e. STAT3-K177 / 180R mutant mice) accompanied by leukocyte and thrombocytosis
[0029] 1. Crispr / Cas9 site design: use CRISPR / Cas9 technology to find the target sequence for the gene structure of the target gene, design guide-RNA for this site according to the target sequence information, and after activity detection, guide-RNA with activity will be RNA and Cas9 were transcribed into RNA in vitro, and guide-RNA, Cas9 mRNA, and oligo donor DNA for recombination were injected into fertilized eggs by microinjection, and positive knockout mice were screened from the offspring.
[0030] According to the above analysis, a guide-RNA (SEQ ID NO.1) was designed for the upstream and downstream sequences of exon No. 6 (82bp), where the mouse mutation site is located. Site 1: The exon6 target sequence is as follows: (SEQ ID NO.4, the underline is the mutation site, the uppercase is exon, the lowercase is intron)
[0031] SEQ...
Embodiment 2
[0050] Example 2: Index detection of STAT3-K177 / 180R mutant mice
[0051] 1. Observation of the phenotype of the mutant mice: observe the surviving STAT3-K177 / 180R mutant homozygous mice, and find that the homozygous mice grow slowly and become smaller ( Figure 4 Middle A), 12 homozygous, 12 heterozygous and wild-type mice were extracted at the age of 12 weeks, and the weight statistics were carried out, which showed that the weight loss of the homozygous mice was statistically significant ( Figure 4 Middle B), after continuous observation of the growth difference between homozygous mice and wild-type mice, it was found that the hair of homozygous mice began to turn yellow and fall off at the later stage of growth, and some hairs turned white, and some even had skin ulcers ( Figure 4 Middle C).
[0052] 2. Spleen index statistics of mutant mice: 12-week-old homozygous (mut / mut) mice and wild-type (wt / wt) mice were randomly selected for dissection, and the spleen of the hom...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com