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Method for constructing plasmids of recombinant human SPINK5 genes and application of plasmids

A construction method and gene technology, applied in the fields of biotechnology and genetic engineering, can solve problems such as unclear role

Pending Publication Date: 2018-08-07
信雅生物科技(苏州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the role of SPINK5 in cutaneous melanoma remains unclear

Method used

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  • Method for constructing plasmids of recombinant human SPINK5 genes and application of plasmids
  • Method for constructing plasmids of recombinant human SPINK5 genes and application of plasmids
  • Method for constructing plasmids of recombinant human SPINK5 genes and application of plasmids

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Embodiment Construction

[0052] A method for constructing a plasmid expressing recombinant human SPINK5 gene, comprising the steps of:

[0053] (1) Using the NCBI (https: / / www.ncbi.nlm.nih.gov / gene) database, obtain the coding (CDS) region sequence (1-1064) of the human SPINK5 gene, the specific sequence is as follows, the underlined ATG is the start codon and TGA is the stop codon.

[0054] ATG AAGATAGCCACAGTGTCAGTGCTTCTGCCCTTGGCTCTTTGCCTCATACAAGGACAATGTGAGAAGGACTCGCTGAGCGAAATGTGCCATGAATTTCAGGCATTTATGAAAAATGGAAAACTGTTCTGTCCCCAGGATAAGAAATTTTTTCAAAGTCTTGATGGAATAATGTTCATCAATAAATGTGCCACGTGCAAAATGATACTTGAAAAAGAAGCAAAATCACAGAAGAGGGCCAGGCATTTAGCAAGAGCTCCCAAGGCTACTGCCCCAACAGAGCTGAATTGTGATGATTTTAAAAAAGGAGAAAGAGATGGGGATTTTATCTGTCCTGATTATTATGAAGCTGTTTGTGGCACAGATGGGAAAACATATGACAACAGATGTGCACTGTGTGCTGAGAATGCGAAAACCGGGTCCCAAATTGGTGTAAAAAGTGAAGGGGAATGTAAGAGCAGTAATCCAGAGCAGGATGTATGCAGTGCTTTTCGGCCCTTTGTTAGAGATGGAAGACTTGGATGCACAAGGGAAAATGATCCTGTTCTTGGTCCTGATGGGAAGACGCATGGCAATAAGTGTGCAATGTGTGCTGAGCTGTTTTTAAAAGAAGCTGAAA...

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Abstract

The invention discloses a method for constructing plasmids of recombinant human SPINK5 genes and application of the plasmids. The method and the application have the advantages that the pathogenesis of melanoma with a high fatality rate is deeply explored, further research and development on novel therapeutic targets for inhibiting malignant behavior such as melanoma cell proliferation and invasion are carried out, and accordingly sufficient laboratory bases can be provided to clinical treatment for the melanoma; interference segments of coding genes SPINK5 of a Lympho-Epithelial Kazal-type related inhibitor (LETKI) are constructed and are applied to the aspects of melanoma cell proliferation and invasion, and accordingly novel experimental evidence can be provided to fundamental researchon the melanoma and research and development on clinical therapeutic targets. The full name of the SPINK5 is Serine Peptidase Inhibitor Kazal Type 5.

Description

technical field [0001] The invention belongs to the field of biotechnology and genetic engineering, and specifically relates to a method for constructing a recombinant plasmid of an epithelial squamous phenotype-related inhibitory protein gene SPINK5 and its application. Background technique [0002] Malignant melanoma has a high mortality rate, is not sensitive to radiotherapy and chemotherapy, and the 5-year survival rate of advanced patients is less than 5%. The incidence and mortality of melanoma in my country are increasing year by year, and the age of onset is younger. Although the emergence of molecular targeted therapy drugs has brought breakthroughs in the clinical treatment of melanoma, unfortunately, CTLA-4 monoclonal antibodies, PD-1 monoclonal antibodies, BRAF inhibitors and MEK inhibitors, etc. None of the therapeutic drugs has been marketed in China, and the price is high, and the drug resistance rate is high. Therefore, it is particularly important to find ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/66A61K48/00A61P35/00
CPCA61K38/57A61K48/005C12N15/66C12N15/85C12N2800/107
Inventor 宋鑫李真董苏伟孟旭东胡红艳
Owner 信雅生物科技(苏州)有限公司
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