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A Gram-stained quality control sheet and its production method and application

A technology of Gram staining and production method, which is applied in the field of Gram staining quality control sheet and its production, can solve the problems of false negative and false positive, achieve the effect of improving quality, improving accuracy, and realizing long-term storage

Active Publication Date: 2020-09-01
CHAOHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem of "false positive" and "false negative" phenomena that are prone to occur due to the inability to accurately judge the color of the staining result when Gram staining is performed on bacteria clinically and experimentally in the prior art, the present invention provides a Gram staining Quality control sheet and its production method and application

Method used

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  • A Gram-stained quality control sheet and its production method and application
  • A Gram-stained quality control sheet and its production method and application
  • A Gram-stained quality control sheet and its production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The application of the Gram-stained quality control sheet of the present embodiment is as follows,

[0060] (1) Pipette the cultured sample bacteria to be tested using an inoculation loop to obtain a sample bacterial liquid;

[0061] (2) Register the information of the sample bacteria in the marking area 1, the information is as follows, Bacillus subtilis CCTCC AB90008, Gram-positive bacteria;

[0062] (3) Select the Gram-stained quality control sheet, and add the sample bacterial liquid dropwise to the sample bacterial area 3 at the same time, then smear, dry and fix the sample bacterial liquid, and then apply the Gram-stained quality control sheet. The protective cover is torn off along the raised edge; perform Gram staining operation on the quality control bacteria area 2 and the sample bacteria area 3 in the Gram staining quality control sheet at the same time, and perform microscopic examination after the Gram staining operation;

[0063] (4) First observe the sta...

Embodiment 2

[0065] The application of the Gram-stained quality control sheet of the present embodiment is as follows,

[0066] (1) Pipette the cultured sample bacteria to be tested using an inoculation loop to obtain a sample bacterial liquid;

[0067] (2) Register the information of the sample bacteria in the marked area 1, the information is as follows, Proteus CCTCC AB 91103, Gram-negative bacteria;

[0068] (3) Select the Gram-stained quality control sheet, and add the sample bacterial liquid dropwise to the sample bacterial area 3 at the same time, then smear, dry and fix the sample bacterial liquid, and then apply the Gram-stained quality control sheet. The protective cover is torn off along the raised edge; perform Gram staining operation on the quality control bacteria area 2 and the sample bacteria area 3 in the Gram staining quality control sheet at the same time, and perform microscopic examination after the Gram staining operation;

[0069] (4) First observe the stained area ...

Embodiment 3

[0071] The application of the Gram-stained quality control sheet of the present embodiment is as follows,

[0072] (1) Pipette the cultured sample bacteria to be tested using an inoculation loop to obtain a sample bacterial liquid;

[0073] (2) Register the information of the sample bacteria in the marking area 1, the information is as follows, Micrococcus luteus CMCC (B) 28001, Gram-positive bacteria;

[0074] (3) Select the Gram-stained quality control sheet, and add the sample bacterial liquid dropwise to the sample bacterial area 3 at the same time, then smear, dry and fix the sample bacterial liquid, and then apply the Gram-stained quality control sheet. The protective cover is torn off along the raised edge; perform Gram staining operation on the quality control bacteria area 2 and the sample bacteria area 3 in the Gram staining quality control sheet at the same time, and perform microscopic examination after the Gram staining operation;

[0075] (4) First observe the sta...

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Abstract

The invention discloses a Gram staining quality control sheet as well as a preparation method and application thereof and belongs to the technical field of staining. According to the invention, the problems that false positive and false negative phenomena are easy to occur due to the fact that a staining result cannot be accurately judged when Gram staining of bacteria is performed in clinical application and experiment in the prior are solved. The invention provides the Gram staining quality control sheet as well as the preparation method and application thereof. The Gram staining quality control sheet comprises a glass slide, a quality control bacterial area, a sample bacterial area and a protection cover. The quality control sheet can effectively avoid the phenomena of false positive and positive negative in the clinical application and the experiment when the Gram staining is carried out on bacteria, and the correctness of the Gram staining result is ensured.

Description

technical field [0001] The invention belongs to the technical field of dyeing, and in particular relates to a Gram staining quality control sheet and a manufacturing method and application thereof. Background technique [0002] In the prior art, "false positive" and "false negative" phenomena are prone to occur in clinical and experimental Gram staining of bacteria, usually due to the quality of Gram staining reagents or improper handling of time in staining, such as decolorization time If it is too long, it is easy to appear "false negative", and if the decolorization time is too short, it is easy to appear "false positive". Clinically, the antibiotics used to treat Gram-positive bacteria are different from those used to treat Gram-negative bacteria, so the correctness of the staining results also has a major impact on doctors' rational prescribing of drugs. [0003] The correctness of the Gram staining experiment is guaranteed by the following methods in the existing meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/14C12Q1/10C12R1/19C12R1/445
CPCC12N1/20C12Q1/10C12Q1/14C12Q2304/10G01N2333/245G01N2333/31
Inventor 方舒张永静钟思夏陶露徐虹高玉荣
Owner CHAOHU UNIV
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