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A 3D printing method for a three-dimensional cell-hydrogel composite structure

A 3D printing and composite structure technology, applied in the field of 3D printing of biomaterials, can solve the problems of cumbersome printing operations, many configuration parameters, and difficult to control well, and achieve low cost of printing biomaterials, excellent mechanical properties, and accurate 3D printing Effect

Active Publication Date: 2019-11-08
艾伯尔三氐打印技术(重庆)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3D printing technology is a combination of computer data model and rapid automatic prototyping system, which processes and superimposes liquid, powder, sheet and other materials layer by layer without additional molds, and finally prints into the desired shape; however, due to 3D printing There are too many configuration parameters in the software, it is difficult to control well, making the determination of the technical parameters of the 3D printer a key factor affecting 3D printing
[0003] At present, it is difficult for 3D printers to print hydrogels to meet the stringent conditions required for 3D printing, such as precise control, biocompatibility, mechanical strength, and rapid prototyping, and the cost of investing in 3D printers and printing hydrogels is relatively high
For example, the prior art CN 106110399 A discloses a 3D printing method of a multi-component composite hydrogel. The flow and velocity of the hydrogel are difficult to program control, the size, thickness and thickness of the printed gel are difficult to accurately control, and the printing accuracy is also difficult. It reaches the micron level; and the printing operation is cumbersome, which greatly reduces the efficiency of 3D printing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A 3D printing method for hydrogel materials. Turn on the sealed printing room and set the UV lamp. Set the UV lamp in the sealed printing room. At the same time, turn on the concentric micro-needle and set the follow-up miniature UV lamp using the 365 UV LED light source, and turn on the 400 times Wireless wifi electron microscope with magnification function, wireless wifi camera remote monitoring and video recording equipment throughout the whole process, specifically include the following steps:

[0021] (1) Preparation of hydrogel cells:

[0022] ①Preparation of positively charged hydrogel: at room temperature, add 12 parts of aminated hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 8 parts of cellulose and stir fully, and finally add 5 parts of cationic chitin and stir Mix well, then vortex the resulting mixture for 30 seconds, and place it in a 37°C water bath for 2 minutes to obtain a positively charged hydrogel;

[0023] ②Preparatio...

Embodiment 2

[0029] A 3D printing method for hydrogel materials. Turn on the sealed printing room and set the UV lamp. Set the UV lamp in the sealed printing room. At the same time, turn on the concentric micro-needle and set the follow-up miniature UV lamp using the 365 UV LED light source, and turn on the 400 times Wireless wifi electron microscope with magnification function, wireless wifi camera remote monitoring and video recording equipment throughout the whole process, specifically include the following steps:

[0030] (1) Preparation of hydrogel cells:

[0031] ①Preparation of positively charged hydrogel: at room temperature, add 8 parts of aminated hyaluronic acid to 27 parts of water and stir to fully dissolve, then drop in 12 parts of cellulose and stir fully, and finally add 3 parts of cationic chitin and stir Mix well, then vortex the obtained mixture for 50 seconds, and place it in a 35°C water bath for 3 minutes to obtain a positively charged hydrogel;

[0032] ②Preparation...

Embodiment 3

[0038] A 3D printing method for hydrogel materials. Turn on the sealed printing room and set the UV lamp. Set the UV lamp in the sealed printing room. At the same time, turn on the concentric micro-needle and set the follow-up miniature UV lamp using the 365 UV LED light source, and turn on the 400 times Wireless wifi electron microscope with magnification function, wireless wifi camera remote monitoring and video recording equipment throughout the whole process, specifically include the following steps:

[0039] (1) Preparation of hydrogel cells:

[0040] ①Preparation of positively charged hydrogel: at room temperature, add 14 parts of aminated hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 6 parts of cellulose and stir fully, and finally add 5 parts of cationic chitin and stir Mix well, then vortex the obtained mixture for 40 seconds, and place it in a water bath at 37°C for 2 minutes to obtain a positively charged hydrogel;

[0041] ②Preparat...

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Abstract

The invention discloses a 3D printing method of a three-dimensional cell hydrogel composite structure. A multi-tube concentric micro-needle printer is adopted to print under a sterile environment; andthe 3D printing method comprises the following steps: (1) solutions of different hydrogel cells are prepared separately; (2) such parameters as the temperature of a 3D printing base plate are set; and (3) after the temperature of the base plate meets set requirements, the prepared solution of one hydrogel cell is pressed in a concentric micro-needle tube of the 3D printer for mixed crosslinking on the base plate under the effect of air pressure to form hydrogel; the solutions of multiple different hydrogel cells are pressed into multiple remaining concentric micro-needle tubes of the 3D printer separately; then, under the set 3D printing parameters, after a layer of hydrogel is printed under the effect of air pressure, a prepared solidifying agent is used for slightly spraying on the layer of hydrogel; and then, the hydrogel and different cells are subjected to mixing crossover to be printed layer by layer according to a preset printing path. The 3D printing method has the advantage of multi-head printing of hydrogel cells.

Description

technical field [0001] The invention relates to a 3D printing method of biological materials, in particular to a 3D printing method of a three-dimensional cell hydrogel composite structure. Background technique [0002] Due to its high water content and extracellular matrix-like characteristics, hydrogel is the preferred substrate for 3D bioprinting and artificial organ preparation, thus becoming a research hotspot in the fields of chemistry, materials and life medicine. 3D printing technology is a combination of computer data model and rapid automatic prototyping system, which processes and superimposes liquid, powder, sheet and other materials layer by layer without additional molds, and finally prints into the desired shape; however, due to 3D printing There are too many configuration parameters in the software, and it is difficult to control well, making the determination of the technical parameters of the 3D printer a key factor affecting 3D printing. [0003] At prese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B29C64/106B29C64/314B29C64/386B33Y10/00B33Y50/00A61L27/52A61L27/22A61L27/20A61L27/02A61L2/10
CPCA61L2/0047A61L27/025A61L27/20A61L27/222A61L27/52A61L2202/21B29C64/106B29C64/314B29C64/386B33Y10/00B33Y50/00C08L5/08C08L1/02C08L5/04
Inventor 杨陈
Owner 艾伯尔三氐打印技术(重庆)有限公司
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