Application of shiitake mushroom volatile oil in preparation of antibacterial drugs or food preservatives
A technology of food preservatives and antibacterial drugs, applied in antibacterial drugs, food preservation, and resistance to vector-borne diseases, etc., can solve problems such as discarding volatile components, wasting resources, and unclear pharmacological effects
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[0020] 1. Materials and Instruments
[0021] 1.1 Materials
[0022] Source of medicinal materials: Dried shiitake mushrooms are from Tongjiang Shanbawang Wild Food Co., Ltd., Sichuan Province.
[0023] 1.2 Reagents
[0024] n-Hexane (Sinopharm Chemical Reagent Co., Ltd., batch number: T20100928); anhydrous sodium sulfate (Sinopharm Chemical Reagent Co., Ltd., batch number: 20140114) was analytically pure; DMSO (dimethyl sulfoxide, Chengdu Kelong Chemical Reagent Factory, Batch number: 2015110201); water is distilled water; MH agar (Hangzhou Best Biotechnology Co., Ltd., batch number: 20160602001). Escherichia coli ATCC25922, Staphylococcus aureus ATCC25923, and ATCC43300 were all donated by Sichuan Antibacterial Research Institute, and the rest of the strains were all from clinical isolation.
[0025] 1.3 Instruments
[0026] Light oil extractor (Sichuan Shuniu Glass Instrument Co., Ltd.), MIT-P bacterial multi-point inoculation instrument (Japan Sakuma Company), electric ...
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