Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer for detecting pseudomonas syringae pv.actinidae of kiwifruit as well as detection method and application thereof

A technology of kiwi fruit and pathogenic bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of easy implementation and simple detection process

Inactive Publication Date: 2018-07-24
绵阳市农业科学研究院 +1
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, as far as the inventor knows, research in this respect is still in a blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer for detecting pseudomonas syringae pv.actinidae of kiwifruit as well as detection method and application thereof
  • Primer for detecting pseudomonas syringae pv.actinidae of kiwifruit as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) DNA is extracted from kiwi fruit; the specific method is:

[0043] 1) Put an appropriate amount of kiwi fruit into a 1.5ml EP tube, add liquid nitrogen, and quickly grind it into powder with a grinding rod;

[0044] 2) Add 550 μL of CTAB extract solution preheated at 65°C, shake vigorously, place in a 65°C incubator for 1 hour, shake and mix once every 10 minutes

[0045] 3) Add 550 μL of chloroform-isoamyl alcohol (24:1 v / v), shake vigorously and mix well, let stand for 10 min, and centrifuge at 13000 r / min for 5 min;

[0046] 4) Take the supernatant, add 600 μL ice ethanol, centrifuge at 13000 r / min for 5 min, pour off the supernatant, and wash the precipitate with 75% ethanol; repeat this step at least once;

[0047] 5) Empty centrifuge at 7500r / min for 3min, suck off excess liquid at the bottom with a pipette, and let it dry naturally at room temperature;

[0048] 6) After drying, add an appropriate amount of sterilized ddH 2 O was fully dissolved, and then s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a group of specific primers for high sensitively detecting pseudomonas syringae pv.actinidae of the kiwifruit. The specific primer consists of a forward primer and a reverse primer, the forward primer is 5'-AAAACCGCTGTGATACAATTCG-3', as shown in SEQ ID NO. 1, and the reverse primer is 5'-GATAGCCCTGAATGGTTTCCGAA-3', as shown in SEQ ID NO.2. The invention also provides a detection method and application based on the specific primer.

Description

technical field [0001] The invention belongs to the technical field of agriculture and plant quarantine, and in particular relates to a high-sensitivity specific primer for detecting kiwifruit canker pathogen Pseudomonas syringae pv. actinidae, a detection method and an application. Background technique [0002] Kiwi fruit is known as the king of fruits and the crown of vitamin C. It has high economic benefits. The main domestic planting areas are Sichuan, Shaanxi and Guizhou. The detection, prevention and control of kiwifruit diseases is a necessary means to improve its economic value and promote the economic development of the kiwifruit industry in the region. [0003] Canker is a fatal bacterial disease that seriously threatens the production of kiwifruit, and has been listed as a national forest plant quarantine object. Pseudomonas syringae is the main pathogenic bacteria of kiwifruit disease, and one of the main pathogenic bacteria is currently identified as Pseudomona...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/38
CPCC12Q1/686C12Q1/689C12Q2565/125
Inventor 石军胡容平黄廷友刘定友项祖芬侍守佩彭涛李春财杨伟张杰
Owner 绵阳市农业科学研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com