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Pelteobagrus vachelli growth related SNP molecular marker and application thereof

A technology of molecular markers for pelagic catfish, applied in the field of genetics and breeding of pelagic catfish, can solve the problems of increased disease, weak disease resistance, slow growth of seedlings, etc., and achieve the effect of promoting the breeding process and convenient operation

Active Publication Date: 2018-07-24
NANJING NORMAL UNIVERSITY
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Problems solved by technology

However, in the process of production, seedlings have slow growth, weak disease resistance, and increased disease germplasm degradation.
However, there is no research report on the SNPs of the IGF-2R gene related to the growth of Pelvis vacheronii.

Method used

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  • Pelteobagrus vachelli growth related SNP molecular marker and application thereof
  • Pelteobagrus vachelli growth related SNP molecular marker and application thereof
  • Pelteobagrus vachelli growth related SNP molecular marker and application thereof

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Embodiment Construction

[0026] The technical solution of the present invention will be described in further detail below through specific examples, but it must be pointed out that the following examples are only used to describe the content of the invention, and do not constitute limitations to the protection scope of the present invention.

[0027] The present invention is based on a pair of primers shown in SEQ ID NO: 2 and SEQ ID NO: 3, through the extraction of the genomic DNA of the peltaceae varskini, PCR amplification, sequencing of the amplified products and analysis of the sequencing results. Two SNP loci (SNPg.7104G>C and SNPg.7170C>A) related to the growth of P. vacheronii have been obtained, which can be applied to molecular marker-assisted breeding of P. vacheronii.

[0028] Below will further illustrate by specific embodiment

[0029] a) the acquisition of the Peltella vacheronii population;

[0030] b) DNA extraction from the caudal fin of the yellow catfish;

[0031] c) based on the...

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Abstract

The invention discloses a pelteobagrus vachelli growth related SNP molecular marker and application thereof. The transcriptome sequence of a SNP molecular marker source gene is shown as SEQ ID NO:1, from the 5' end, the two SNP loci SNPg.7104G>C and SNPg.7170C>A exist at the 7104th and 7170th sites of the sequence. The SNP loci disclosed by the invention can be used for molecular marker assisted breeding, are not limited by the age, sex and the like of pelteobagrus vachelli, can be used for early breeding of pelteobagrus vachelli, and can significantly promote the breeding process of pelteobagrus vachelli, and the method is accurate and reliable, and is easy to operate.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the growth of the yellow catfish and its application, and belongs to the field of genetic breeding of the yellow catfish. Background technique [0002] Pelteobagrus vachelli, also known as Pelteobagrus vachelli, is the largest individual species in the genus. , is also an important economic fish in the Yangtze River Basin. Because of its delicious taste, tender meat, and no intermuscular thorns, it is deeply loved by consumers and farmers. It is an important freshwater famous economic fish in my country and has a good breeding prospect. However, in the process of production, seedlings have slow growth, weak disease resistance, and increased disease germplasm degradation. The problem of improved species has become one of the main factors restricting the stable development of the peltaceae aquaculture industry. Therefore, cultivating new strains with excellent growth traits such as fast growth...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 尹绍武李杰王涛张国松
Owner NANJING NORMAL UNIVERSITY
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