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Production method of three-enzymolysis one-fermentation physical therapy liquid

A production method and physiotherapy technology, applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, medical raw materials derived from arthropods, etc., can solve the problems of reduced curative effect, loss of active ingredients, unfavorable absorption, etc., and achieve rheumatic pain relief, retain full effect

Inactive Publication Date: 2018-07-20
安徽精准医疗产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the physiotherapy solutions for treating rheumatic diseases are to extract the active ingredients from insects and grasses by water extraction or decoction, and then prepare corresponding external liquid preparations. Although such extraction methods are simple, they are effective during the extraction process. There is a lot of loss of components, and the protein components of the extracted insect medicines are severely damaged and are not conducive to the absorption of the human body, resulting in a decrease in curative effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The production method of three-enzyme one-fermentation physiotherapy solution comprises the steps:

[0027] S1: Prepare raw materials according to the following proportions by weight: 4 parts of scorpions, 5 parts of silkworms, 2 parts of leeches, 5 parts of centipedes, 4 parts of black snakes, 8 parts of sarcophagus, 6 parts of fenugreek, and 4 parts of snow lotus 12 parts of wormwood leaves, 4 parts of astragalus;

[0028] S2: Mix and crush the raw materials until the particle size is below 100 mesh, add distilled water and soak for 45 minutes according to the ratio of material to liquid 1g: 10mL, prepare the raw material liquid, and use HAc to adjust the pH of the system to 6.5, then add cellulase and papain and neutral protease at 55°C for 5 hours; the added amounts of cellulase, papain, and neutral protease are 1200U / g, 650U / g, and 800U / g, respectively, and the added amounts here are enzymes added to raw materials After being submerged in the solution, the activit...

Embodiment 2

[0032] The production method of three-enzyme one-fermentation physiotherapy solution comprises the steps:

[0033] S1: Prepare raw materials according to the following proportions by weight: 6 parts of scorpion, 2 parts of silkworm, 1 part of leech, 8 parts of centipede, 4 parts of black snake, 5 parts of sinensis, 10 parts of osmanthus, 3 parts of snow lotus 11 parts of Artemisia argyi leaves, 3 parts of Astragalus membranaceus;

[0034] S2: Mix and crush the raw materials until the particle size is below 100 mesh, add distilled water and soak for 60 minutes according to the ratio of material to liquid 1g:15mL, prepare the raw material liquid, and use HAc to adjust the pH of the system to 6.0, then add cellulase and papain and neutral protease at 50°C for 6 hours; the added amounts of cellulase, papain and neutral protease were 1000U / g, 500U / g and 1000U / g, respectively;

[0035] S3: After the enzymolysis is completed, put the enzymolysis liquid into the fermenter, and insert...

Embodiment 3

[0038] The production method of three-enzyme one-fermentation physiotherapy solution comprises the steps:

[0039] S1: Prepare raw materials according to the following proportions by weight: 3 parts of scorpions, 5 parts of silkworms, 1 part of leeches, 8 parts of centipedes, 3 parts of black snakes, 10 parts of sinensis, 6 parts of osmanthus, and 4 parts of snow lotus 12 parts of Artemisia argyi leaves, 6 parts of Astragalus membranaceus;

[0040] S2: Mix and pulverize the raw materials until the particle size is below 100 mesh, add distilled water and soak for 30 minutes to prepare a raw material solution, and use HAc to adjust the pH of the system to 6.3, then add cellulase, papain and neutral protease at 65°C Enzymolysis for 3 hours; the added amounts of cellulase, papain, and neutral protease were 1500U / g, 800U / g, and 700U / g, respectively;

[0041] S3: After the enzymolysis is completed, put the enzymolysis liquid into the fermenter, and insert the viable bacteria into 5...

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PUM

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Abstract

The invention discloses a production method of three-enzymolysis one-fermentation physical therapy liquid. The production method comprises the following steps: matching scorpion, stiff silkworms, leeches, centipedes, zaocys, herba lycopodii, herba speranskiae tuberculatae, herba saussureae involucratae, folium artemisiae argyi and radix astragali to serve as raw materials; crushing the raw materials; performing enzymolysis on the raw materials through cellulase, papain and neutral protease; fermenting the raw materials which are subjected to the enzymolysis through bacillus subtilis, and preparing to obtain the physical therapy liquid. The physical therapy liquid has a good relieving and treating effect on the pain from rheumatism.

Description

technical field [0001] The invention relates to a production method of a three-enzyme and one-fermentation physiotherapy liquid. Background technique [0002] Rheumatic diseases are a group of extremely common clinical syndromes with arthralgia and fear of wind and cold as the main symptoms. group of diseases. [0003] At present, most of the physiotherapy solutions for treating rheumatic diseases are to extract the active ingredients from insects and grasses by water extraction or decoction, and then prepare corresponding external liquid preparations. Although such extraction methods are simple, they are effective during the extraction process. The loss of components is large, and the protein components of the extracted insect medicines are severely damaged and are not conducive to the absorption of the human body, thereby reducing the curative effect. Contents of the invention [0004] In order to solve the above-mentioned technical problems, the invention provides a p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61P29/00A61K35/648A61K35/646A61K35/64A61K35/62A61K35/583
CPCA61K9/0014A61K35/583A61K35/62A61K35/63A61K35/646A61K35/648A61K36/11A61K36/185A61K36/282A61K36/285A61K36/47A61K36/481A61K2236/19A61K2236/331A61K2300/00
Inventor 不公告发明人
Owner 安徽精准医疗产业研究院有限公司
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