Pest repellent containing methyl myristate and preparation and use method thereof
A technology of methyl tetradecanoate and pest repellent, which is applied in the directions of animal repellent, botanical equipment and method, pest repellent, etc., to achieve the effects of realizing pollution-free control, avoiding control difficulties and high efficiency
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[0031] A preparation method of a pest repellent, comprising the steps of:
[0032] (1) Preparation of mother liquor A: Accurately weigh 0.87-8.70 g of methyl myristate and dissolve it in a solvent;
[0033] (2) Preparation of auxiliary liquid B: prepare a sodium dodecylbenzenesulfonate solution with a mass fraction of 10% with deionized water;
[0034] (3) Take 10mL solution B and solution A, mix them with ultrasonic waves for 4~6 minutes;
[0035] (4) Dilute the volume to 1 L with a solvent to obtain the pest repellent.
[0036] The method of using the pest repellent is to dilute the pest repellent by 0-5 times with ethanol or water, and spray it evenly on the flower buds, branches and leaves of the plants directly.
[0037] The pests are beet armyworm and / or cotton bollworm.
[0038] The beet armyworm is the female beet armyworm.
[0039] The pest repellent of the present invention can be used for the prevention and control of pests such as beet armyworm and cotton bollw...
Embodiment 1
[0043] Example 1: Discovery of Pest Repellent Substances
[0044] (1) Feeding method of beet armyworm
[0045] Put the mature larvae of beet armyworm back into a 250 mL beaker with moist sand and seal it with gauze. After pupation and eclosion, the adults were transferred to another 250 mL beaker. Supplement nutrition with 10% honey water to make eggs and hatch on healthy Chinese rose leaves, and raise them to mature larvae (feeding conditions: 27°C, 70% RH, 12 h photoperiod). Let it pupate in the same way, take out the pupae and distinguish between male and female under a stereoscope, and raise the male and female pupae separately, and use them for biological tests after eclosion.
[0046] (2) Inhibitory effect of host plants infected with powdery mildew on oviposition behavior of beet armyworm adults
[0047] Put about 3 cm thick sterilized soil in two 500 ml beakers, and sprinkle appropriate amount of sterile water to moisten the soil. Select 6 fresh branches of healthy ...
Embodiment 2
[0055] Example 2: Isolation and Identification of Repellent Active Components
[0056] The headspace dynamic adsorption method was used to extract the volatile component mixture of Chinese rose leaves infected with powdery mildew, and the mixture was analyzed and tested by gas chromatography-antenna electrophysiology (GC-EAD). The results are as follows image 3 , Figure 4 , Table 1 shows. The monomeric compounds that can produce obvious EAD signals in the mixture were further identified by GC-MS. Among them, the No. 3 peak is methyl myristate, and the content of this compound in the mixture is relatively large, and the EAD signal generated is relatively strong. The results show that the natural compound (mixture) extracted from the susceptible rose plants contains methyl myristate (monomer), which may be the sensitive host for the female beet armyworm moth to identify the spawning site One of the important information compounds.
[0057] Table 1 Volatile components of su...
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