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Enzymic uric acid detection agent with high interference preventing capacity

A technology of detection reagent and enzymatic method, which is applied in the field of enzymatic uric acid detection reagents, can solve the problems of use interference, low detection results, and high requirements for personnel operation, and achieve the effect of removing inhibition

Active Publication Date: 2018-07-17
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The three methods of voltammetry, phosphotungstic acid reduction, and capillary electrophoresis are rarely used in clinical practice; while the two methods of liquid chromatography and isotope dilution mass spectrometry have the advantages of high accuracy and strong specificity. The requirements for instruments are high, the detection cost is high, and the operation requirements for personnel are also very high. Only large hospitals or research institutes can use it; with the continuous improvement of domestic enzyme extraction technology and the improvement of the stability of enzyme products, Enzymatic detection of blood urea has made great progress and has become the most widely used method for detecting blood uric acid in clinical practice. Low-level results or even zero or negative values ​​seriously affect clinical detection and application
Because the blood components of newborns are very complex, there are samples different from normal people, and the samples of newborns often have problems such as jaundice and hemolysis, which lead to interference in the use of conventional enzymatic UA detection reagents.
Therefore, according to the problem of poor anti-interference ability of enzymatic detection of blood UA, an enzymatic UA detection reagent with strong anti-interference ability was invented.

Method used

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  • Enzymic uric acid detection agent with high interference preventing capacity
  • Enzymic uric acid detection agent with high interference preventing capacity
  • Enzymic uric acid detection agent with high interference preventing capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A kind of existing enzymatic UA detection reagent and detection method:

[0051] Reagent R1

[0052] Phosphate buffer 100mmo1 / L

[0053] 4-Aminoantipyrine 1.2mmo1 / L

[0054] Peroxidase 2KU / L

[0055] Reagent 2 (R2):

[0056] Phosphate buffer 100mmo1 / L

[0057] DHBS 8mmo1 / L

[0058] Uricase 1000U / L.

[0059] The usage method of this embodiment reagent:

[0060] An existing enzymatic UA detection reagent described in this example uses a fully automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 fully automatic analyzer, etc., and uses the two-point endpoint method for determination. Place R1 and R2 on the corresponding reagent position according to the ratio of 4:1, and place distilled water, standard and sample on the corresponding position of the sample plate, the operation is as follows figure 1 .

Embodiment 2

[0062] A newly invented enzymatic UA detection reagent with strong anti-interference ability, characterized in that it includes reagent R1 and reagent R2, and the reagent components are as follows:

[0063] Reagent 1 (R1):

[0064] Piperazine-1,4-diethanesulfonic acid buffer 20mmo1 / L

[0065] 4-Aminoantipyrine 1.2mmo1 / L

[0066] Sodium nitrite 4mmol / L

[0067] Lithium chloride 5mmol / L

[0068] Ascorbate oxidase 3KU / L

[0069] Thiourea 1mmol / L

[0070] Bovine serum albumin 1g / L

[0071] Lauryl Trimethyl Betaine 1ml / L

[0072] Triton-305 1ml / L

[0073] MIT.HCL 0.2g / L

[0074] Reagent 2 (R2):

[0075] Tris Buffer 100mmo1 / L

[0076] F-DAOS (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxy-4-fluoroaniline) 12.5mmo1 / L

[0077] Uricase 1000U / L

[0078] Bovine serum albumin 1g / L

[0079] Peroxidase 5KU / L

[0080] MIT.HCL 0.2g / L.

[0081] The usage method of this embodiment reagent:

[0082] The reagents in this example are used with a fully automatic biochemical analyze...

Embodiment 3

[0084] This example describes a newly invented enzymatic UA detection reagent with strong anti-interference ability and increased key raw materials, including reagents R1 and R2, and its components are as follows:

[0085] Reagent 1 (R1):

[0086] Piperazine-1,4-diethanesulfonic acid buffer 20mmo1 / L

[0087] 4-Aminoantipyrine 1.2mmo1 / L

[0088] Sodium nitrite 8mmol / L

[0089] Lithium chloride 10mmol / L

[0090] Ascorbate oxidase 5KU / L

[0091] Thiourea 2mmol / L

[0092] Bovine serum albumin 5g / L

[0093] Lauryl Trimethyl Betaine 3ml / L

[0094] Triton-305 3ml / L

[0095] MIT.HCL 0.5g / L

[0096] Reagent 2 (R2):

[0097] Tris Buffer 100mmo1 / L

[0098] F-DAOS (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxy-4-fluoroaniline) 12mmo1 / L

[0099] Uricase 2000U / L

[0100] Bovine serum albumin 15g / L

[0101] Peroxidase 10KU / L

[0102] MIT.HCL 0.5g / L.

[0103] The usage method of this embodiment reagent:

[0104] The reagents in this example are used with a fully automatic ...

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Abstract

The invention relates to an enzymic uric acid detection agent with high interference preventing capacity. The agent is characterized in that an agent R1 includes a buffering solution, 4-ampyrone, sodium nitrite, an ion balancing agent, ascorbic acid oxidase, a heavy metal ion chelator, bovine serum albumin, dodecyl trimethyl betaine, triton-305 and a preservative; an agent R2 includes a bufferingsolution, F-DAOS (N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3, 5-dimethoxy-4-fluoroaniline), uricase, bovine serum albumin, peroxidase, a preservative, and other components. The agent is high in interference preventing capacity and particularly capable of accurately detecting a sample of a newborn.

Description

technical field [0001] The invention relates to an enzymatic uric acid detection reagent with strong anti-interference ability, belonging to the technical field of clinical in vitro detection. Background technique [0002] Uric acid (UA) is the final product of purine metabolism. After purine is produced in the body, it will be oxidized again in the liver to 2,6,8-trioxypurine, also known as uric acid. It has the functions of scavenging oxygen free radicals and anti-oxidation. But it also has the effect of stimulating smooth muscle proliferation. Uric acid is one of the main non-protein nitrogen (NPN) metabolites in the blood. Under normal circumstances, there are about 1200 mg of uric acid in the body, and about 600 mg of uric acid is newly produced every day, and 600 mg is excreted at the same time, which is in a balanced state. However, if there is too much uric acid in the body to be excreted or the uric acid excretion mechanism degrades, the body will retain too much u...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/5308
Inventor 罗维晓胡晓飞王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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