Liver microfluidic control chip capable of realizing two-dimensional and three-dimensional cross co-culture and application thereof
A co-culture, liver technology, applied in the field of microfluidics, can solve the problems of lack of bile circulation pathway, inability to simulate sinus space structure, lack of
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[0017] This embodiment adopts figure 1 Structure, the material of the upper cover and the lower cover is selected from Agree, the material of the upper substrate, the middle substrate and the lower substrate is selected from PDMS polymer; the material of the upper porous membrane and the lower porous membrane is selected from polycarbonate, and the upper substrate is cultivated The chamber is a cylinder with a diameter of 6mm and a height of 200um; the middle substrate culture chamber is a cylinder with a diameter of 6mm and a height of 500um; the lower substrate culture chamber is a cylinder with a diameter of 6mm and a height of 200um. The fluid of the upper substrate flows into the substrate through the upper cover plate, and then divides into two destinations, one is connected to the outside of the chip through the channel of the upper substrate, and the other enters the lower substrate through the upper porous membrane, the middle substrate, and the lower porous membrane; ...
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