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Lactobacillus additive and application, and preparation method thereof

A technology of lactic acid bacteria and additives, applied in the field of microorganisms, can solve the problems of not being able to meet the market demand and single species of lactic acid bacteria, achieve the effects of reducing the production of ammonia nitrogen, simple and convenient method, and improving fermentation quality

Active Publication Date: 2018-07-10
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microorganisms and convert limited sugar, reduce the pH value of silage, and improve the quality of silage. Different silage materials have different fermentation characteristics, and the current lactic acid bacteria suitable for silage are single, which cannot meet market demand

Method used

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  • Lactobacillus additive and application, and preparation method thereof
  • Lactobacillus additive and application, and preparation method thereof
  • Lactobacillus additive and application, and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0050] The preparation method of the above-mentioned lactic acid bacteria additive is obtained by mixing the components of the lactic acid bacteria additive.

[0051] The preparation method of above-mentioned lactic acid bacteria additive also comprises: 1) get pasture sample, add sterile distilled water, dilute to 10 -1 , 10 -3 and 10 -5 Sample dilutions of three dilutions; draw sample dilutions and apply to MRS solid medium for culture, and subculture twice continuously to obtain purified colonies;

[0052] 2) After culturing the purified colony obtained in step 1) on a lactic acid bacteria solid medium at 37° C. under constant temperature and anaerobic conditions for 24 hours, carry out the identification of lactic acid bacteria through Gram staining and catalase contact reaction, and identify The final lactic acid bacteria were added to the liquid nutrient medium containing sterilized glycerin, and stored at -80°C to obtain isolated and purified lactic acid bacteria;

...

Embodiment 2

[0065] Physiological and biochemical assays: The physiological and biochemical characteristics of Pediococcus pentosaceae, Weissella ansinusphaera and Lactobacillus plantarum in Example 1 were analyzed respectively, and the results are shown in Table 3.

[0066] Different temperature test: the Pediococcus pentosaceae, Weissella annulus and Lactobacillus plantarum in the embodiment 1 were respectively inoculated in the MRS liquid medium with an inoculum size of 3%, and cultivated under the temperature conditions of Table 2, Observe its growth status, and the results are shown in Table 3.

[0067] Salt tolerance test: the Pediococcus pentosaceae in embodiment 1, Weissella annulus and Lactobacillus plantarum were treated with the MRS liquid culture medium containing NaCl concentration 3.0% (w / v) and 6.5% (w / v) respectively After culturing at 30°C for 2 days, the salt tolerance was measured, and the results are shown in Table 3.

[0068] Acid-resistant test: after Pediococcus pen...

Embodiment 4

[0081] Using the lactic acid bacteria additive of the present invention as pasture silage additive

[0082] Take forage grass with a height of 1.5-2m at the Chongzhou base of Sichuan Agricultural University, cut it into a length of about 20mm, and obtain fresh samples. Each treatment takes 500g of fresh samples and puts them into plastic bags (25×35cm; Aodeju, China). 1 Inoculate the obtained lactic acid bacteria additive into the MRS liquid medium, and use sterilized distilled water as the blank control, and the number of live bacteria of the obtained strain is set as 1×10 6 CFU / gFW, to the strain additive solution; spray the bacterial strain additive solution evenly into the forage sample, mix evenly, pack the silage in a bag, vacuum seal it, leave it at room temperature (27-37°C) for fermentation for 60 days, and then unpack it to obtain silage Sample.

[0083] 1. Sensory evaluation:

[0084] 1) Silage samples According to the sensory silage scoring standard and grade eva...

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Abstract

The invention discloses a lactobacillus additive. The lactobacillus additive comprises a component A and a component B, a volume ratio of the component A to the component B is (1-2):(1-2), and the component A is one selected from pediococcus pentosaceus, weissella cibaria and lactobacillus plantarum, the component B is one selected from pediococcus pentosaceus, weissella cibaria and lactobacillusplantarum, the component A and the component B are different, the lactobacillus additive is a grass silage additive, quality of a grass silage feed is increased, and the lactobacillus additive has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactic acid bacteria additive and its application and preparation method. Background technique [0002] Silage is a storage method that converts the fermentation substrate in raw materials into acidic substances such as lactic acid through the proliferation of lactic acid bacteria, and maintains an acidic anaerobic environment to facilitate the long-term preservation of silage crops. Silage is yellow-green in color, sour in smell, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding, and has become an important source of high-quality roughage. [0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmfu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/01
CPCA23K30/18C12N1/20
Inventor 闫艳红关皓张新全李小铃帅杨李小梅黄琳凯聂刚汪霞黄婷
Owner SICHUAN AGRI UNIV
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