Weissella cibaria as well as application and screening inspection method thereof
A technology of Weissella sinensis and colony, applied in the field of microorganisms, can solve the problems of not meeting the market demand, single variety of lactic acid bacteria, etc., and achieve the effects of improving palatability, reducing fiber content and improving fermentation quality
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Embodiment 2
[0048] Physiological and biochemical assays: analyze the physiological and biochemical characteristics of Weissella esophagus in Example 1, and the results are shown in Table 2.
[0049] Different temperature test: the Weissella esophagus in Example 1 was inoculated into the MRS liquid medium with an inoculum size of 3%, cultured under the temperature conditions in Table 2, and its growth status was observed. The results are shown in Table 2.
[0050] Salt tolerance test: with the Weissella esophagus in embodiment 1 in the MRS liquid culture medium that contains NaCl concentration 3.0% (w / v) and 6.5% (w / v), after cultivating at 30 ℃ for 2 days, measure Its salt tolerance, the results are shown in Table 2.
[0051] Acid resistance test: After culturing the Weissella anthurium in Example 1 in the MRS liquid medium with the pH value in Table 2 at 30° C. for 7 days, the acid resistance was measured, and the results are shown in Table 2.
[0052] Sugar fermentation test: The chara...
Embodiment 3
[0065] Using Weissella phalloides of the present invention as pasture silage additive
[0066] Forage grass with a height of 1.5-2m was collected from the Chongzhou base of Sichuan Agricultural University, cut into a length of about 20mm, and fresh samples were obtained. For each treatment, 300g of fresh samples were taken and put into plastic bags (25×35cm; Aodeju, China). Weissella esophagus of the present invention in example 1 1.0 * 10 8 Add 1ml of CFU / g fresh material to each bag of silage, then vacuum seal it, place it at room temperature to ferment, each treatment group has 3 repetitions, and open the bag after 60 days of silage to obtain silage samples.
[0067] 1. Sensory evaluation:
[0068] 1) Silage samples According to the sensory silage scoring standard and grade evaluation compiled by the German Farming Association (DLG) in 1899, sensory scores were performed on the smell, structure and color of the silage.
[0069] Table 4 Sensory Evaluation Criteria for Sila...
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