Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of SNP (Single Nucleotide Polymorphism) sites of yak whole genome, primer set for detecting and kit

A whole genome and primer set technology, applied in the field of animal husbandry, achieves the effects of easy standardization and automatic detection, and high determination accuracy

Active Publication Date: 2018-07-06
INST OF ANIMAL SCI & VETERINARY TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI +2
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide the application of 16 SNP sites in the whole genome of yaks in the analysis of yak phenotypic traits or molecular breeding, so as to solve the problem of using SNP sites in the prior art to analyze yak phenotypic traits or molecules. Problems with Breeding Analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of SNP (Single Nucleotide Polymorphism) sites of yak whole genome, primer set for detecting and kit
  • Application of SNP (Single Nucleotide Polymorphism) sites of yak whole genome, primer set for detecting and kit
  • Application of SNP (Single Nucleotide Polymorphism) sites of yak whole genome, primer set for detecting and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example provides a method for selecting the site with the highest reliability of the SNP mutation from the resequencing data of 96 yaks to prepare a chip.

[0038] First, the Illumina X Ten sequencing platform was used to perform whole-genome resequencing data (10X coverage for each individual) from 32 different local yak populations (3 individuals in each population, a total of 96 yak individuals) from all over China, and 2592G the sequencing data. On this basis, a quality control is first performed to filter out data with low sequencing quality. Next, we use the BWA comparison software to compare the total reads to the reference genome, generate a comparison result file in sam format, and then use samtools to convert the result file in sam format into bam format and sort it. Finally, we use the identification SNP most prevalent in GATK to identify SNP mutations in 96 yaks. Finally, we selected some high-confidence sites from the 96 SNP mutations of yaks to make...

Embodiment 2

[0051] This example provides a screening method for 268 SNP sites of yak meat production-related traits.

[0052] A total of 268 individuals from 3 nationally certified yak populations, including Jiali yak, Pali yak and Sibu yak, were selected, and SNP sites were screened by the chip prepared in Example 1. The samples were strictly selected yak individuals aged 4-9 in the young adults in each group, and healthy individuals with low body weight and high body weight were screened in each group as samples for microarray screening. The body weight data of each individual was recorded in detail, and GWAS analysis was performed based on the obtained chip screening data, and finally 16 loci most related to yak meat production were found.

[0053] The specific steps are:

[0054]Sample gDNA was quantified using NanoDrop ND-2000 (Thermo Scientific) and DNA integrity was detected by gel electrophoresis. After passing the DNA quality inspection, sample amplification, fragmentation, pre...

Embodiment 3

[0061] This embodiment provides a primer set for detecting the 16 SNP sites screened in Example 1, and the corresponding relationship between the nucleotide sequence of the primer set and the SNP site is shown in the following table:

[0062]

[0063]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of 16 SNP (Single Nucleotide Polymorphism) sites of a yak whole genome in yak phenotypic character or molecular breeding analysis, aiming at solving the problem inthe prior art that the yak phenotypic character or molecular breeding analysis is not carried out by adopting the SNP sites. Nucleotide sequences of the 16 SNP sites provided by the invention are shown as SEQ ID No. 1 to SEQ ID No. 16. The invention further discloses a primer set for detecting the 16 SNP sites and a kit comprising the primer set. The invention further discloses a yak phenotypic character or molecular breeding analysis method. The 16 SNP sites provided by the invention can provide supports for an upstream and downstream breeding process and have the advantages of high determination accuracy, easiness for realizing standard and automatic detection and the like.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to the application of SNP sites in the whole genome of yaks, a primer set and a kit for detection. Background technique [0002] Yak is a unique breed of cattle distributed in the center of the Qinghai-Tibet Plateau and its adjacent alpine and sub-alpine regions. It can make full use of the forage resources of the alpine grassland and has strong adaptability to the ecological environment conditions of the alpine grassland. Living freely under the harsh environmental conditions of thin air, short grass growth period, cold, and long dry grass period, they reproduce and provide local herdsmen with necessities for production and life such as milk, meat, wool, labor force, and fuel. Indispensable, what can be called "all-around" livestock. Yak is an extremely valuable gene pool in terms of genetic resources. However, the number and variety of yaks in China are larg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/16C12Q2600/124C12Q2600/156
Inventor 姬秋梅钟金城信金伟柴志欣庞剑会
Owner INST OF ANIMAL SCI & VETERINARY TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com