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Preparation method of Xinjiang black bee royal jelly globulin enzymolysate with antioxidant activity

A technology of antioxidant activity and enzymatic hydrolysis products is applied in the research field of Xinjiang black bee royal jelly globulin, which can solve the problems of single research method and so on.

Active Publication Date: 2018-07-06
TARIM UNIV
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Problems solved by technology

The current research on the antioxidant properties of royal jelly is limited to the research and inference of its overall components, and the research methods are relatively simple

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  • Preparation method of Xinjiang black bee royal jelly globulin enzymolysate with antioxidant activity
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  • Preparation method of Xinjiang black bee royal jelly globulin enzymolysate with antioxidant activity

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Embodiment Construction

[0048] In order to illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments and accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.

[0049] 1 Materials and methods

[0050] 1.1 Materials and reagents

[0051] Material: Xinjiang black bee royal jelly.

[0052] Reagent: petroleum ether; NaCl; 75wt% ethanol (AR); NaOH (AR); diphenyl-2-picryhydrazyl free radical (DPPH free radical); 85wt% phosphoric acid; bovine serum albumin (crystallization); Formaldehyde (Tianjin Zhiyuan Chemical Reagent Co., Ltd.); Trypsin (Gade Chemical Tokyo Synthesis Industry Co., Ltd.); Pepsin (Gade Chemical Tokyo Synthesis Industry Co., Ltd.); Trypsin (Gade Chemical Tokyo Synthes...

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Abstract

The invention discloses a preparation method of a Xinjiang black bee royal jelly globulin enzymolysate with antioxidant activity. The preparation method comprises the following steps: (1), preparing lyophilized Xinjiang black bee royal jelly powder; (2), degreasing, and preparing royal jelly main protein; (3), preparing globulin; (4), preparing the Xinjiang black bee royal jelly globulin enzymolysate; (5), separating peptide fragments with different molecular weights from the globulin enzymolysate. The globulin is extracted from Xinjiang black bee royal jelly and then enzymolyzed to obtain theenzymolysate, and the enzymolysate is divided into the peptide fragments with different molecular weights; the antioxidant activity of the royal jelly globulin and the enzymolysate thereof is measured by a determination method of scavenging diphenyl-2-picrylhydrazyl radicals (DPPH.) and a method of inhibiting linoleic acid oxidation, the total antioxidant capacity is measured, and a peptide substance with the strongest antioxidant activity being lower than 3kD is obtained.

Description

technical field [0001] The invention relates to the research on the royal jelly globulin of Xinjiang black bee. More specifically, it relates to a method for preparing an enzymatic hydrolyzate of royal jelly globulin from Xinjiang black bee royal jelly with antioxidant activity. Background technique [0002] Xinjiang black bee, also known as Yili black bee, belongs to the same strain as European black bee, and is one of the four famous bees in the world. It is suitable for living in Xinjiang, but due to its limited number, it is now on the verge of extinction and is listed as a protected animal, so it has high economic and scientific value. Xinjiang black bees are mainly distributed in Yili, Tacheng, Altay, Xinyuan, Turks, Nileke, Zhaosu, Gongliu, Yining and Burqin in Xinjiang. Its main raw material is non-polluting flower secretions deep in the Tianshan Mountains at an altitude of 1800m-2500m. The main nutrients are minerals, organic acids, proteins, vitamins and enzymes....

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 姜建辉赵俭波丁慧萍辛文博高来福
Owner TARIM UNIV
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