Centrifugal type micro-fluidic chip

Abstract

The invention provides a centrifugal type micro-fluidic chip which is simple and reasonable in structure and saved in processing cost. The centrifugal type micro-fluidic chip comprises a substrate anda cover plate closely matched with the substrate, wherein the end surface of the substrate matched with the cover plate is provided with a plurality of reaction holes distributed in a circumferentialdirection and at least one main channel distributed in the circumferential direction and communicated with the plurality of reaction holes; each main channel is a wavy channel with a plurality of wave crests and a plurality of wave troughs; each reaction hole is provided with a connecting channel facing towards the center of the substrate; the connecting channels are communicated with the wave troughs of the main channels; the reaction holes are communicated with the main channels through the connecting channels; and the main channel between every two adjacent reaction holes is provided witha liquid sealing unit. The centrifugal type micro-fluidic chip provided by the invention has the advantages that based on the liquid sealing principle of a U-shaped tube, the reaction holes after centrifugal distribution are ensured to be full of liquid, reaction products in all reaction holes are prevented from diffusing into the adjacent reaction holes, the flow speed of the liquid in the main channels is uniform, and more uniform distribution of samples is realized.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a centrifugal microfluidic chip, belonging to the fields of life science and medical detection. Background technique [0002] Microfluidic chip technology integrates the basic operation units of sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a micron-scale chip, and automatically completes the entire analysis process. Due to its great potential in the fields of biology, chemistry, and medicine, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, and machinery. Microfluidic chip technology provides a variety of implementation platforms for single-molecule nucleic acid detection methods. The analysis of biochemical samples is integrated on a microfluidic chip of several square centimeters through micromachining technology. Using a small centrifug...

AI Technical Summary

Problems solved by technology

However, the channel section design of the chip’s main channel with narrow peaks and wide troughs affects the flow rate of the liquid in the main channel, which cannot completely solve the problem of uniform distribution of samples and the generation of bubbles in the main channel, and increases the complexity of chip manufacturing and processing costs.

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