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Method and device for processing circulating tumor DNA repetitive sequence

A technology of DNA repeating and repeating sequences, applied in the field of genetic engineering, can solve problems such as low accuracy, and achieve the effect of improving processing accuracy

Active Publication Date: 2018-06-29
GENE CRAB BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Embodiments of the present invention provide a method and device for processing repetitive sequences of circulating tumor DNA, so as to at least solve the technical problem of low accuracy in the processing method of sequencing data in the prior art to delete or mark repetitive sequences in sample sequencing

Method used

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  • Method and device for processing circulating tumor DNA repetitive sequence
  • Method and device for processing circulating tumor DNA repetitive sequence

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Embodiment 1

[0046] According to an embodiment of the present invention, an embodiment of a method for processing circulating tumor DNA repeat sequences is provided. It should be noted that the steps shown in the flowcharts of the accompanying drawings can be implemented in a computer system such as a set of computer-executable instructions and, although a logical order is shown in the flowcharts, in some cases the steps shown or described may be performed in an order different from that shown or described herein.

[0047] figure 1 It is a flowchart of a method for processing circulating tumor DNA repeat sequences according to an embodiment of the present invention, such as figure 1 As shown, the method includes the following steps:

[0048] Step S102, obtaining the sequencing data and the reference genome sequence of the circulating tumor DNA to be detected, wherein the sequencing data is the data obtained by high-throughput sequencing of the circulating tumor DNA to be detected, and the...

Embodiment 2

[0193] According to an embodiment of the present invention, an embodiment of a device for processing circulating tumor DNA repeat sequences is provided.

[0194] Figure 4 It is a schematic diagram of a processing device for circulating tumor DNA repeat sequences according to an embodiment of the present invention, such as Figure 4 As shown, the device includes:

[0195] The obtaining module 42 is used to obtain the sequencing data and the reference genome sequence of the circulating tumor DNA to be detected, wherein the sequencing data is the data obtained by high-throughput sequencing of the circulating tumor DNA to be detected, and the sequencing data includes: multiple pairs of double-end sequences.

[0196] Specifically, the above-mentioned circulating tumor DNA to be detected can be the gene sequence extracted from the patient's blood, lymph fluid, interstitial fluid, cerebrospinal fluid and other body fluids. In the embodiment of the present invention, ctDNA extracted...

Embodiment 3

[0206] According to an embodiment of the present invention, an embodiment of a storage medium is provided. The storage medium includes a stored program, wherein when the program is running, the device where the storage medium is located is controlled to execute the above-mentioned method for processing circulating tumor DNA repeat sequences.

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Abstract

The invention discloses a method and device for processing a circulating tumor DNA repetitive sequence. The method comprises the steps that sequencing data and a reference genome sequence of to-be-detected circulating tumor DNA are obtained, wherein the sequencing data is obtained by carrying out high-throughput sequencing on the to-be-detected circulating tumor DNA and comprises a plurality of pairs of double-ended sequences; the sequencing data is compared with the reference genome sequence to obtain a first comparison result, wherein the first comparison result at least comprises genome positions, base sequences and corresponding base quality value sequences of the double-ended sequences; the type of each pair of double-ended sequences is determined on the basis of the first comparisonresult, wherein the types comprise independent sequences and repeated sequences. The method and the device solve the problem that a sequencing data processing method in the prior art is used for sequencing samples to delete or mark repetitive sequences and accordingly the accuracy is low.

Description

[0001] technical field [0002] The invention relates to the field of genetic engineering, in particular to a method and device for processing circulating tumor DNA repeat sequences. Background technique [0003] During the process of division and proliferation, tumor cells will undergo apoptosis, death, and necrosis, and will also actively release DNA fragments carrying tumor mutations into body fluids, that is, circulating tumor DNA (circulating tumor DNA, ctDNA for short), which mostly exists in In body fluids such as blood, synovial fluid and cerebrospinal fluid, especially in plasma free DNA (cell-free DNA, cfDNA). Through the sequencing of ctDNA, the genomic region where the base sequence changes (mutation) occurs on the DNA molecule of the tumor cell is detected, which can effectively reflect the patient's response to the treatment; after the drug response is detected, the tumor may become resistant to the drug treatment, ctDNA detection can also track the generation o...

Claims

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Application Information

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IPC IPC(8): G06F19/22
CPCG16B30/00
Inventor 郭昊于佳宁韩天澄宋雪林小静
Owner GENE CRAB BIOTECH CO
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