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Detection method of glycerol in beer

A technology of medium glycerol and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable environmental protection, high toxicity, high detection cost, etc., and achieve the effect of cost saving, safety and environmental protection detection cost

Inactive Publication Date: 2018-06-29
TSINGTAO BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing methods for detecting glycerol by liquid chromatography, the amino column method is mostly used, and the mobile phase uses acetonitrile, which is highly toxic, unfavorable for environmental protection and high in detection costs.

Method used

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  • Detection method of glycerol in beer
  • Detection method of glycerol in beer
  • Detection method of glycerol in beer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Establishment of standard working curve:

[0024] Accurately weigh 2.0905 g of standard glycerol (purity 100%) with an electronic balance, add ultrapure water to stir and dissolve, and set the volume to 25 mL to obtain a glycerol solution with a concentration of ①83.62 g / L. Use a pipette gun to measure different volumes of this solution and mix it with ultrapure water to obtain a progressively diluted glycerin solution with a concentration of ②41.81g / L (dilution 2 times), ③27.87g / L (3 times) , ④20.91g / L (4 times), ⑤13.94g / L (6 times), ⑥6.97g / L (12 times) glycerin solution. Dilute the glycerol solutions with concentrations of ⑤13.94g / L and ⑥6.97g / L by 12 times to obtain glycerol solutions with concentrations of ⑦1.162g / L and ⑧0.58g / L.

[0025] The above 8 glycerol solutions with concentration gradients were sequentially injected for detection. With the concentration of glycerol as the abscissa, and the chromatographic peak area response as the ordinate, the linear r...

Embodiment 2

[0048] Select the brewing process of IPA beer, from yeast addition to cold storage. According to the above method, the content of glycerol in the beer fermentation process was tracked, and the detection results are shown in Table 4.

[0049] Table 4

[0050]

[0051]

[0052] Combined with the data in Table 4, it can be seen that from the addition of yeast until the fifth day, the content of glycerol has been continuously rising, and then basically maintained a stable state. It can be seen that the method provided by the present application can effectively detect the content of glycerol in the beer sample.

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Abstract

The invention provides a detection method of glycerol in beer and belongs to the technical field of beer detection. The method is safe and environmentally friendly and has relatively good accuracy andrepeatability; the method can be used as an effective detection means for regulation and control of beer production. The detection method provided by the invention comprises four steps of preparing astandard working solution, preparing a sample solution, carrying out liquid chromatographic analysis and standard curve drawing and calculating a result. The detection method provided by the invention can be applied to detection of the glycerol in the beer.

Description

technical field [0001] The invention belongs to the technical field of beer detection, in particular to a method for detecting glycerol in beer. Background technique [0002] The content of glycerol in beer is second only to ethanol and carbon dioxide. It is an important flavor substance that constitutes the body of beer, and it has sweetness and viscosity. Glycerol can also work synergistically with other flavor substances to offset bad tastes such as astringency, and endow beer with full, mellow and harmonious characteristics. In addition, glycerol also plays an important role in regulating the osmotic pressure of yeast cells and maintaining the redox balance of the cytoplasm. Due to its importance to beer flavor and fermentation process, glycerol is necessary to detect it. [0003] For the detection of glycerol in beer, these methods such as density method, titration, and capillary gas chromatography can be applied to the inspection of pure glycerol, but the purity requ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 余俊红尹花李梅陈华磊杨朝霞闫鹏张宇昕田玉红杨梅
Owner TSINGTAO BREWERY
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