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Finger-print spectrum detection method for Biling Weitong granules and finger-print spectrum thereof

The technology of a fingerprint spectrum and a detection method is applied to the fingerprint spectrum detection method of Biling Weitong granules and the field of fingerprint spectrum, and achieves the effects of reduced possibility, high precision and simple method.

Active Publication Date: 2018-06-29
YANGTZE RIVER PHARMA GRP JIA NGSU LONGFENGTANG TRADITIONAL CHINESE MEDICINE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are 11 medicinal materials in Piling Weitong Granules. In the current quality standard, it is not enough to measure the content of berberine hydrochloride, the main component, and it is necessary to control its substance group as a whole.

Method used

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  • Finger-print spectrum detection method for Biling Weitong granules and finger-print spectrum thereof
  • Finger-print spectrum detection method for Biling Weitong granules and finger-print spectrum thereof
  • Finger-print spectrum detection method for Biling Weitong granules and finger-print spectrum thereof

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Experimental program
Comparison scheme
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Embodiment

[0041] The method for constructing the fingerprint of Piling Weitong Granules uses liquid chromatography to measure Piling Weitong Granules. The specific method is as follows:

[0042] (1) Preparation of reference substance solution:

[0043] Take an appropriate amount of gallic acid, chlorogenic acid, naringin, hesperidin, berberine hydrochloride, palmatine hydrochloride, coptisine hydrochloride, and dehydrocorydalline, and add methanol to make it contain 80 μg of gallic acid and chlorogenic acid per 1ml A mixed reference solution of 40 μg of acid, 30 μg of naringin, 15 μg of hesperidin, 20 μg of berberine hydrochloride, 5 μg of palmatine hydrochloride, 10 μg of coptisine hydrochloride, and 40 μg of dehydrocordyrine was obtained.

[0044] (2) Preparation of the test solution:

[0045] Take the Piling Weitong granule to be tested, grind it finely, take about 1.0g, accurately weigh it, put it in a 50ml conical flask with a stopper, add 25ml of 30vol% methanol precisely, seal i...

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Abstract

The invention discloses a finger-print spectrum detection method for Biling Weitong granules. The method is established by an efficient liquid chromatography; chromatographic conditions are as follows: octadecylsilane bonded silica is taken as a filling agent, acetonitrile is taken as a mobile phase A; a 0.01mol / L potassium dihydrogen phosphate (phosphoric acid is used for adjusting the pH to 2 to4) water solution is taken as a mobile phase B; gradient elution is performed; the flow velocity is 0.5 to 1.5ml.min<-1>;the column temperature is 25 to 40 DEG C; the detection wave length is 210 to360nm; the number of theoretical plates is not less than 2,000 according to berberine hydrochloride peak. The detection method disclosed by the invention has the advantages of high analysis speed, high efficiency and high repeatability. The singleness and one-sidedness of the existing quality control are avoided, so that the quality of the Biling Weitong granules can be evaluated more comprehensively and accurately, and the method is of great significance to the clinical efficacy. The invention further provides a Biling Weitong granules fingerprint spectrum obtained through the finger-print spectrum detection method for the Biling Weitong granules particles.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for detecting fingerprints of Piling Weitong granules and the fingerprints thereof. Background technique [0002] Piling Weitong Granules have the functions of promoting qi and blood circulation, harmonizing stomach and relieving pain. It is used to treat epigastric pain caused by qi stagnation and blood stasis, and chronic superficial gastritis with the above symptoms. [0003] Piling Weitong Granule is a master of traditional Chinese medicine, an academician of the Chinese Academy of Engineering, and a consulting expert of the Central Health Bureau, Professor Dong Jianhua. Years of clinical practice experience, summed up the empirical prescription for the treatment of gas stagnation and blood stasis type epigastric pain and chronic gastritis caused by mixed cold and heat, which is a national special gastric medicine for the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 宋敏曾海松李敏沈芸董阗伟闫述模李伟吴革林曹泽庆
Owner YANGTZE RIVER PHARMA GRP JIA NGSU LONGFENGTANG TRADITIONAL CHINESE MEDICINE CO LTD
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