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Cocktail type cryoprotectant and application thereof

A cryoprotectant and cocktail technology, applied in the field of biomedicine, can solve problems such as inability to meet requirements, achieve high feasibility and application promotion value, inhibit the formation of ice crystals, and improve the effect of survival rate

Active Publication Date: 2018-06-29
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of DMSO in combination with trehalose, ethylene glycol, and hydroxyethyl starch can improve the cryopreservation effect to a limited extent, but it still cannot meet the requirements

Method used

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  • Cocktail type cryoprotectant and application thereof
  • Cocktail type cryoprotectant and application thereof
  • Cocktail type cryoprotectant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The present embodiment provides a kind of cocktail type cryoprotectant, it comprises following components:

[0038]

[0039] This embodiment also provides an application of the above-mentioned cocktail cryoprotectant in slow cryopreservation of human embryonic stem cells.

Embodiment 2

[0041] The present embodiment provides a kind of cocktail type cryoprotectant, it comprises following components:

[0042]

[0043] This embodiment also provides an application of the above-mentioned cocktail cryoprotectant in vitrification cryopreservation of human induced pluripotent stem cells.

Embodiment 3

[0045] The present embodiment provides a kind of cocktail type cryoprotectant, it comprises following components:

[0046]

[0047] This embodiment also provides an application of the above-mentioned cocktail cryoprotectant in vitrification cryopreservation of human embryonic stem cells.

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PUM

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Abstract

The invention relates to the field of biological medicines and in particular to cocktail type cryoprotectant and application thereof. The cocktail type cryoprotectant comprises the following components: 1-60% (w / v) of a polysaccharide, 1-50% (w / v) of an amino acid, 1-20% (w / v) of a polyhydric alcohol and 0-10% (w / v) of a hydroxyl-containing macromoleclar polymer. The polysaccharide, the amino acid, the polyhydric alcohol and the hydroxyl-containing macromoleclar polymer of the cryoprotectant are relatively low in toxicity with high concentrations, formation of intracellular ice crystals can beeffectively inhibited with the polysaccharide, the amino acid, the polyhydric alcohol and the hydroxyl-containing macromoleclar polymer of the proportions, cryopreserved cells can be alleviated fromdamage caused by low temperature environments, the survival rates of the cryopreserved cells after anabiosis culture can be increased, the toxicity of the cryopreserved cells can be greatly reduced, and thus the cocktail type cryoprotectant provided by the invention has very high security feasibility and application and popularization values.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a cocktail type cryoprotectant and its application. Background technique [0002] Human pluripotent stem cells (hPSCs) include human embryonic stem cells and human induced pluripotent stem cells. The acquisition of such cells marks a new era in the field of biomedical research. Human pluripotent stem cells have the ability of self-renewal and can differentiate into almost all types of human tissue cells. These excellent characteristics make hPSCs have broad application prospects in the fields of tissue engineering, regenerative medicine and cell transplantation, and it is expected to produce a series of cell therapies for the treatment of Parkinson's disease, diabetes, cardiovascular and other diseases. However, the low recovery rate of hPSCs after cryopreservation seriously affects and hinders their wide application in biological and medical fields. [0003] Cell cryopreservation is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 饶伟刘静
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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