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Application of GAD67 and epigenetic mark thereof which serve as early marker of fetal-original bipolar affective disorder susceptibility

A technology for bipolar disorder and early warning, applied in the field of gene function and application

Active Publication Date: 2018-06-22
WUHAN UNIV
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The above content suggests that the DNA methylation level of leukocytes in the blood may be used as a biomarker of susceptibility to fetal-derived diseases. At present, no research has linked the expression and methylation of GAD67 in leukocytes to bipolar disorder

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  • Application of GAD67 and epigenetic mark thereof which serve as early marker of fetal-original bipolar affective disorder susceptibility
  • Application of GAD67 and epigenetic mark thereof which serve as early marker of fetal-original bipolar affective disorder susceptibility
  • Application of GAD67 and epigenetic mark thereof which serve as early marker of fetal-original bipolar affective disorder susceptibility

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Embodiment 1

[0043] 1. Animals and Handling

[0044] 20 male Wistar rats (body weight 260-300g), and 40 female Wistar rats (body weight 200-240g). Provided by Hubei Provincial Animal Center. Free to drink and eat, after 7 days of adaptive feeding, according to male: female = 1:2 cage, vaginal smear in the next morning, to determine the pregnant rats, recorded as pregnancy 0 day. The pregnant rats were randomly divided into two groups: the control group and the experimental group (ethanol exposure group, PEE). The pregnant rats in the experimental group were treated with 4 g / kg·d ethanol by intragastric administration from GD9 to GD20, while the control group was given the same volume of saline. Part of the rats in each group were sacrificed on GD20. Female and male fetuses were separated and weighed, and IUGR was diagnosed by weighing. Fetal blood was collected for serum preparation and leukocyte extraction, and the fetal hippocampus and hypothalamus were isolated under a microscope. ...

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Abstract

The invention discloses application of GAD67 and an epigenetic mark thereof which serve as early markers of fetal-original bipolar affective disorder susceptibility, and belongs to the field of gene functions and application. Research results show that in hippocampi and white blood cells of fetal rats in a pregnancy ethanol exposure group, compared with a normal control group, the methylation level of a GAD67 promoter region is significantly reduced, and the gene expression of the GAD67 promoter region is improved; after growing up, the rats in the xenobiotic exposure group show high hypothalamic-pituitary-adrenal (HPA) axis stress sensibility and bipolar affective disorder susceptibility under the condition of chronic unpredictable stress. Therefore, risks that offspring have bipolar affective disorder after growing up can be judged by detecting the epigenetic modification and expression levels of the GAD67 genes in the white blood cells of fetal blood / umbilical cord blood. Research bases are provided for an early warning technology of the fetal-original bipolar affective disorder, and meanwhile, possibilities are provided for effectively treating the fetal-original bipolar affective disorder in early stages.

Description

technical field [0001] The invention belongs to the field of gene function and application, and relates to a glutamic acid decarboxylase 67 (Glutamatedecarboxylase 67, GAD67) gene and its epigenetics as an early warning biomarker for susceptibility to fetal-derived bipolar disorder. Background technique [0002] Depression and anxiety bipolar disorder refers to the coexistence of depression and anxiety. It is not severe enough to be diagnosed as depression, nor can it be completely diagnosed as anxiety. It is a coexisting state in the middle of depression and anxiety. It has long been recognized that environmental factors can lead to the occurrence of mental illness, and now people start from bipolar disorder and the hypothalamic-pituitary-adrenal (HPA) axis, cytokines, circadian rhythm, oxidative stress, etc. Predisposing causes of psychiatric problems were assessed and a strong genetic basis for bipolar disorder was proposed [1]. Studies have confirmed that the adverse in...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/154C12Q2600/158
Inventor 徐丹汪晖文印宪焦哲潇黄松强
Owner WUHAN UNIV
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