Method for extracting enteric microbial genome DNA

Abstract

The invention discloses a method for extracting enteric microbial genome DNA. The method specifically comprises the following steps: (1) coprophilous fungi sample collection; (2) thallus acquisition;and (3) coprophilous fungi genome DNA extraction and purification. According to the method disclosed by the invention, usage of an organic solvent is reduced, the cost is low, and the extracted microbial total DNA has high purity; guanidinium isothiocyanate is capable of rapidly breaking cells and inhibiting ribozyme released from the cells, and the obtained DNA has high quality; enriched DNA population information can be obtained, the diversity and community composition of enteric microorganisms can be comprehensively reflected, the operating steps are less, the stability is excellent, and the method is a practical and reliable fecal microorganism DNA extraction method and can be widely applied to study on intestinal microecology.

Description

technical field [0001] The invention relates to related fields such as scientific research on intestinal microorganisms, clinical diagnosis, drug development, etc., in particular to a method for extracting intestinal microorganism genome DNA. Background technique [0002] The intestinal tract is colonized with a large number and variety of intestinal flora, which form a mutually beneficial symbiotic relationship with the host, which has a major impact on the health of the host. In recent years, with the continuous research on the regulation of intestinal flora In-depth, it is found that the intestinal flora not only regulates intestinal activities, but also affects the brain function and behavior of the host. Therefore, the physiological health of the human body is not only regulated by its own genes, but also affected by the intestinal flora. There are 1000-1150 species of bacteria in the human intestinal tract, of which 160 are dominant species, and there are different typ...

AI Technical Summary

Problems solved by technology

Due to the particularity of gut microbial genome research, the composition of feces is complex and affected by various factors, the purity of genomic DNA obtained is not high, resulting in instability of downstream experiments such as genome sequencing data

In recent years, relevant institutions at home and abroad have compared and evaluated the genomic DNA extraction methods of intestinal flora, and found that there are many problems in the existing methods, such as some undigested food residues, polysaccharides, urea and digestive enzymes, including The components will affect the subsequent PCR analysis, the DNA extraction efficiency is generally low, and the stability is poor

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