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Immobilized activating enzyme for promoting food nutrition absorption and preparation technology

A technology for nutrient absorption and enzyme activation, applied in the direction of glycosylation enzymes, biochemical equipment and methods, immobilized on or in inorganic carriers

Inactive Publication Date: 2018-06-19
BEIJING HUANERKANG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of immobilized activated enzymes and preparation processes for promoting the absorption of food nutrients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Put the three-necked flask with 800mL of deionized water in a constant temperature water bath at 65°C, feed nitrogen into the three-necked flask at a gas flow rate of 110L / h for 40 minutes, and then add 6.4 grams of FeSO 4 ·7H 2 O, 4.1 g FeCl 3 ·6H 2 O and 2.3 g FeCl 2 4H 2 O, stirred at 1000r / min for 15 minutes, cooled to room temperature to obtain the mixed solution H1;

[0055] (2) 50 ml of NH 3 ·H 2 O was added to the mixed solution H1, shaken in a shaker at a temperature of 45°C for 15 minutes, then shaken in a shaker at a temperature of 80°C for 30 minutes, and cooled to room temperature to obtain a mixed solution H2;

[0056] (3) Using a magnet to magnetically separate the mixed solution H2, discarding the supernatant to obtain a mixture A;

[0057] (4) Add mixture A to 400mL deionized water, stir at 1000r / min for 15 minutes, then use a magnet for magnetic separation, and discard the supernatant to obtain mixture A1;

[0058] (5) Add mixture A1 to 400...

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Abstract

The invention discloses an immobilized activating enzyme for promoting food nutrition absorption. The immobilized activating enzyme is prepared by the following steps: preparing a mixture A from FeSO4.7H2O, FeCl3.6H2O, FeCl2.4H2O and NH3.H2O; washing the mixture A, and treating with a mixed solution prepared from hydroxypropylcyclodextrin, potassium alginate and sodium caseinate, to obtain a mixture C; washing the mixture C, and treating with a mixed solution prepared from chitosan and ammonium persulfate, to obtain a mixture D; washing the mixture D, and treating with a mixed solution prepared from aluminum ammonium sulfate, polyvinyl pyrrolidone, glutaraldehyde and lactase so as to obtain a substance which is the immobilized activating enzyme for promoting food nutrition absorption. In the invention, the prepared immobilized activating enzyme for promoting food nutrition absorption has the characteristics of high activity, strong adaptability, long service life, convenience in separation, reutilization, etc.

Description

technical field [0001] The invention belongs to the field of food biotechnology, in particular to an immobilized activated enzyme for promoting food nutrition absorption and a preparation process. Background technique [0002] Lactase, also known as β-galactosidase, can catalyze the hydrolysis of lactose into galactose and glucose to produce low-lactose milk. At the same time, lactase has the ability to transglycoside, which can connect galactose to lactose to generate galactooligosaccharides, which can be used as additives in other foods. The use of lactase hydrolyzed milk to make yogurt saves time and the amount of sucrose compared with ordinary skim milk, and can improve the flavor and taste of yogurt, because lactose is decomposed into glucose and galactose, which increases the sweetness of yogurt , and lactic acid bacteria can directly use glucose and galactose to speed up acid production. In the process of making milk-flavored bread, adding lactase hydrolyzed milk ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10
CPCC12N9/2471C12N11/10C12N11/14C12Y302/01023
Inventor 扈佳玉
Owner BEIJING HUANERKANG TECH DEV
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