Method for detecting grain insectsby use of nuclear magnetic resonance technology

A technology of nuclear magnetic resonance and technical detection, which is applied in the direction of analysis by nuclear magnetic resonance, can solve the problems of impracticality, poor detection effect, and high detection cost, and achieve good market application prospects, simple operation, and high detection accuracy. Effect

Inactive Publication Date: 2018-06-15
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the biophoton emission is to detect the spontaneous photons of the organism. Due to the different biological tissue structures of hidden eggs or larvae, the number of spontaneous photons is also different. Therefore, the detection of the number of biophotons can be used theoretically, but this method High-precision biophoton number detection equipment is required, and environmental interference is difficult to remove, because it is not easy to implement and has poor practicability. It is still in the theoretical research stage
The acoustic method is to detect and identify pests through the sound of pests gnawing on grains. Since this type of sound is extremely

Method used

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  • Method for detecting grain insectsby use of nuclear magnetic resonance technology
  • Method for detecting grain insectsby use of nuclear magnetic resonance technology
  • Method for detecting grain insectsby use of nuclear magnetic resonance technology

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The method for detecting insect pests in a certain wheat grain storage depot using nuclear magnetic resonance technology, the steps are as follows:

[0023] S1: Randomly collect wheat grains that have not been infested by pests in the grain depot as a standard product, take about 1 g of the standard product and put it in an NMR test tube. The height of the standard product in the NMR test tube is controlled at 2.2-2.5 cm. The product was detected by low-frequency NMR, and the spin-spin relaxation time T of the standard product was measured by CPMG pulse sequence 2 , the test conditions for low-frequency NMR detection are: sequence parameter settings: sampling points TD=249612, main frequency SW=200KHZ, cumulative times NS=4, echo time Echo Count=5000, participating inversion points 200, relaxation time points 100, the number of iterations is 1000000;

[0024] S2: Randomly take the wheat grains in the grain depot as the samples to be tested (excluding the wheat grains w...

Embodiment 2

[0029] With reference to the method of Example 1, another wheat grain storage depot in different regions was selected to test, and the following test results were obtained:

[0030]

[0031] Similarly, it can be seen from the above results that the relaxation time T of the moisture in the wheat grain of the sample to be tested 21 , T 22 , T 23 Compared with the uninfected standard, there were significant differences. In order to improve the reliability, the combination of dissection method and dyeing method was used for verification. The results showed that there were egg stage pests and larval stage pests in the wheat grains of the samples to be tested.

[0032]Proved by the above examples, adopt the detection method of grain pest situation provided by the present invention, utilize nuclear magnetic resonance technology, can accurately judge whether the grain of the grain sample to be tested is infiltrated by pests, thereby judge whether there is grain pest in the stored...

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Abstract

The invention belongs to the technical field of grain insect detection, and specifically discloses a method for detecting grain insects by use of nuclear magnetic resonance technology. The method fordetecting the grain insects is as follows: first using insect non-infested stored grain particles as a standard product, performing low-frequency nuclear magnetic resonance testing on the standard product, using a CPMG pulse sequence to determine the spin-spin relaxation time T2 of the standard product; then taking a sample to be tested for low-frequency nuclear magnetic resonance testing, and using the CPMG pulse sequence to determine the spin-spin relaxation time T2 of the sample to be tested; and analyzing data of the spin-spin relaxation time T2 of the standard product and data of the spin-spin relaxation time T2 of the sample to be tested, when the data of the spin-spin relaxation time T2 of the standard product and the data of the spin-spin relaxation time T2 of the sample to be tested are significantly different, insect eggs, larvae or pupaes in the to-be-detected stored grain sample particles can be determined, namely, the to-be-detected stored grain has the insects. The methodfor the grain detecting insects has the advantages of being fast and simple, high in precision and good in repeatability.

Description

technical field [0001] The invention relates to the technical field of grain insect pest detection, in particular to a method for detecting grain insect pest using nuclear magnetic resonance technology. Background technique [0002] Food is the material basis for human survival and an indispensable industrial raw material, and it plays an important role in the national economy. However, in the long-term storage of grain, the stored grain is inevitably affected by environmental factors, which will cause a large number of pests and molds to grow and metabolize toxins, resulting in deterioration of grain quality and endangering food safety and human health. According to the preliminary estimate of the post-harvest grain loss survey by the World Joint Food and Agriculture Organization, the losses caused by stored grain pests account for about 50% of the stored grain losses. Among stored grain pests, moth-eating pests are the most harmful to grain reserves. Borering stored grai...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 张玉荣
Owner HENAN UNIVERSITY OF TECHNOLOGY
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