Method for identifying Aoectochilus formosanus cultivated in different cultivation manners

A calculation method and technology for tissue culture seedlings, which are applied in measuring devices, material analysis by optical means, instruments, etc., can solve the problems of insignificant differences, chaotic sales market, hindering the healthy development and scale expansion of the clematis industry, etc. Achieving good application prospects

Inactive Publication Date: 2018-06-15
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The identification of clematis in different cultivation methods on the market is limited to empirical judgment, but the difference between 3-month-old bottle seedlings, 4-month-old bottle seedlings, or 6-month-old seedlings planted in the soil is not significant, leading some unscrupulous merchants to directly sell Tissue-cultured bottle seedlings were put on the market, shoddy as good ones, causing confusion in the sales market, and seriously hindering the healthy development and scale expansion of the clematis industry

Method used

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  • Method for identifying Aoectochilus formosanus cultivated in different cultivation manners
  • Method for identifying Aoectochilus formosanus cultivated in different cultivation manners
  • Method for identifying Aoectochilus formosanus cultivated in different cultivation manners

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Effect test

Embodiment 1

[0025] Embodiment 1 Detection method of the present invention

[0026] (1) Sample treatment: wash the fresh samples to be tested in batches for 3 to 5 times until the water becomes clear and there is no sediment on the surface of the samples, place them in a 60°C oven to dry to constant weight, crush them through a 60-mesh sieve, and take an appropriate amount for each batch. Put it into the finger tube and tamp it so that the sample height is between 2cm, to be tested;

[0027] (2) Spectrum acquisition and preprocessing: scan the sample with NIRFlex N-500 near-infrared spectrometer, acquisition parameters: the scanning range is 4000-10000cm -1 , the number of scans is 32, and the resolution is 8cm -1 , each sample is reloaded and scanned 3 times. Before each scan, the sample in the finger tube should be tamped to make the sample even and flat. The system will automatically store the NIRS spectrum of the scanned sample into the spectral database; in the NIRCal 5.4 data proces...

Embodiment 2

[0033] Embodiment 2 detection method of the present invention

[0034] (1) Sample treatment: wash the fresh samples to be tested in batches for 3 to 5 times until the water becomes clear and the surface of the samples is free of sediment, put them in a 55°C oven and dry them to constant weight, crush them through a 100-mesh sieve, and take an appropriate amount for each batch. Put it into the vial and tamp it down so that the height of the sample is between 1cm, to be tested;

[0035] (2) Spectrum acquisition and preprocessing: scan the sample with NIRFlex N-500 near-infrared spectrometer, acquisition parameters: the scanning range is 4000-10000cm -1 , the number of scans is 32, and the resolution is 8cm -1 , each sample is reloaded and scanned 3 times. Before each scan, the sample in the finger tube should be tamped to make the sample even and flat. The system will automatically store the NIRS spectrum of the scanned sample into the spectral database; in the NIRCal 5.4 data ...

experiment example 1

[0042] Experimental example 1 Identification of different cultivation methods of Taiwan golden clematis

[0043] 1 Experimental materials

[0044] 1.1 Instrument

[0045] NIRFlex N-500 near-infrared spectrometer, solid measuring cell, accessories for 6-position vial, sample rotator diffuse reflection integrating sphere, NIRCal 5.4 software for data processing and modeling (Buchi Labortechnik AG company).

[0046] DF-15 desktop continuous feeding pulverizer (Wenling Linda Machinery Co., Ltd.), 60-mesh standard inspection sieve (60-mesh aperture, 0.3mm, Shangyu Yinhe Test Instrument Factory), DHG-9030H constant temperature oven (Shanghai Jinghong Experimental Equipment Ltd.).

[0047] 1.2 Experimental samples

[0048] Collect 10 batches of 3-month-old bottle seedlings, 4-month-old bottle seedlings, and 6-month-old planting seedlings; the samples come from Nanjing Geyuan Agriculture and Forestry Technology Co., Ltd. The cut-off point is 3 months for 3-month-old bottle seedlin...

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Abstract

The invention discloses a method for identifying Aoectochilus formosanus cultivated in different cultivation manners based on near-infrared spectroscopy. The method can be used to identify a three-month bottle Aoectochilus formosanus seedling, a four-month bottle Aoectochilus formosanus seedling or a six-month Aoectochilus formosanus seedling planted in soil. The method includes the following steps: (1) taking a sample powder to be tested, loading the sample, and acquiring a near-infrared spectrum; and (2) preprocessing the near-infrared spectrum by adopting a PCA-Cluster analysis technology as a calculation method and selecting a smooth second-order derivation technology (dg2) and the standard normal variation (SNV), and identifying the Aoectochilus formosanus cultivated in different cultivation manners by using the score of the main component of the sample. The identifying method has the advantages of simplicity, fastness, accuracy, greenness, environmental protection, and harmlessness to the sample, can effectively identify the three-month bottle Aoectochilus formosanus seedling, the four-month bottle Aoectochilus formosanus seedling or the six-month Aoectochilus formosanus seedling planted in soil, and has a good application prospect.

Description

technical field [0001] The invention relates to a method for identifying Taiwan golden clematis in different cultivation ways. Background technique [0002] Anoectochilus roxburghii is a plant of the genus Orchidaceae, mainly distributed in Fujian and Taiwan regions of my country, and is a rare and endangered perennial medicinal material. Auranthus clematis has the effect of clearing away heat and cooling blood, dispelling wind and dampness, and it is mainly used to treat diseases such as nephritis, cystitis, diabetes, bronchitis, and convulsions in children. Clematis has high medicinal and health value, but wild resources are scarce. Currently, the market is dominated by cultivated products, and the circulating strains mainly include pointed leaf, large round leaf, small round leaf, Hongxia and Taiwan golden clematis. The cultivation methods are mainly divided into tissue culture and planting in the soil. Most of the tissue culture products are tissue culture bottle seedli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563
CPCG01N21/359G01N21/3563
Inventor 黄泽豪张勋陈抒云林羽许文徐伟
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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