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Method for identifying litchi honey

A technology of lychee honey and vitex honey, applied in the field of food science, can solve the problems of high market price, rainy flowering period, damage to consumers' interests, etc.

Inactive Publication Date: 2018-06-12
NORTHWEST UNIV(CN) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of pollen, the flowering period of lychee honey is rainy, the year is different, the output is not stable, and the market price is relatively high
In this context, the emergence of some inferior honey and even adulterated honey has caused the quality of lychee honey on the market to vary, seriously damaging the interests of consumers.

Method used

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  • Method for identifying litchi honey
  • Method for identifying litchi honey
  • Method for identifying litchi honey

Examples

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Embodiment Construction

[0027] Further description will be given below in conjunction with the accompanying drawings and embodiments.

[0028] 10 batches of litchi honey (L1-L10), Vitex twig honey (J1-J10) and rapeseed honey (Y1-Y10) samples were weighed 5 g each, fully dissolved with 30 mL of hydrochloric acid aqueous solution with a pH of 2, and directly injected into the Amberlite XAD−2 type adsorption resin chromatography column (25 cm × 2.5 cm), waited for the sample to be completely adsorbed at a slow flow rate, then rinsed with 75 mL of aqueous hydrochloric acid at pH 2, 100 mL of distilled water, and then rinsed with 100 mL of distilled water. mL of pure methanol for elution. After the eluted methanol solution was concentrated under reduced pressure and vacuum at 40 °C, the volume was adjusted to 2 mL with chromatographically pure methanol to obtain the test sample, which was stored at 4 °C until injection.

[0029] time

[0030] Establish the HPLC-DAD fingerprints of litchi honey,...

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Abstract

The invention discloses a method for identifying litchi honey. The method comprises the following steps: firstly, constructing an HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detection) fingerprint chromatogram of litchi honey, chaste honey and rape honey so as to determine characteristic markers of the litchi honey; then, separating and purifying the determined characteristic markers according to a preparative liquid chromatography so as to respectively obtain two kinds of characteristic markers with higher purity; and finally, analyzing the molecule information of the characteristic markers by using a quadrupole / time-of-flight mass spectrometer so as to finally determine that the molecular weight of the two kinds of characteristic markers is 264 and the molecular formulais C15H20O4, thereby conjecturing that the two kinds of characteristic markers are isomers. The method disclosed by the invention is reasonable in design, and the selected analytical instrument is high in sensitivity, high in analysis speed, high in separation efficiency and highstrong in feasibility and provides a basis for authenticity identification and quality control of the litchi honey.

Description

technical field [0001] The invention relates to a method for identifying lychee honey, which belongs to the field of food science. Background technique [0002] Honey (honey) is a kind of natural substance brewed by bees through their own enzymes and special substances after collecting plant nectar or its secretions. The main components in honey are glucose, fructose and water, and the sum of their mass accounts for more than 80% of the honey mass. In addition, honey is also rich in active substances, such as phenolic acids, flavonoids, alkaloids and so on. The content of these active substances is closely related to the biological activities of honey, including anti-oxidation, anti-tumor, antibacterial and anti-inflammatory, and protection of cardiovascular and cerebrovascular. The types and contents of active ingredients of honey are different from the flower sources of honey. Therefore, the types of honey can be identified according to the characteristic markers of hone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14
Inventor 曹炜贺琼程妮吕新刚吕焕明
Owner NORTHWEST UNIV(CN)
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