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Preparation method of conductive nano-silver wire patterned film

A patterned thin film, conductive nano technology, applied in the direction of biochemical equipment and methods, general culture methods, microbial measurement/testing, etc., to achieve the effect of huge economic value and social value

Active Publication Date: 2018-06-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has never been attempted for stem cell research and tissue engineering applications

Method used

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Experimental program
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Embodiment 1

[0024] A method for preparing a conductive nano-silver wire patterned film, the film is based on chitosan, and the nano-silver wires are evenly distributed on the surface of the chitosan according to the pattern; the patterned pattern can be freely designed according to experimental requirements; the used The patterned mold is a light-transmitting and breathable polydimethylsiloxane polymer PDMS microfluidic chip with a pattern structure, and the ratio of polydimethylsiloxane polymer PDMS monomer to initiator is 10:1;

[0025] The preparation method requirements of the conductive nano-silver wire patterned film are:

[0026] First design the required patterned mask according to the requirements, and make the SU-8 template: pour 10:1 polydimethylsiloxane polymer PDMS on the SU-8 template, and heat it in an oven at 80°C for 20-40min Curing; after that, the cured PDMS is peeled off from the template and cut into an appropriate size for later use;

[0027] Punch holes at the liqu...

Embodiment 2

[0040] Design the required patterned mask according to the experimental requirements, and make the SU-8 template. In this embodiment, the designed serpentine channel chip is taken as an example. Pour 10:1 PDMS on the SU-8 template, heat it in an oven at 80°C for 30 minutes to cure, peel off the cured PDMS from the template, cut it into an appropriate size, and punch it at the liquid inlet and outlet of the PDMS chip channel. hole. Clean the surface with alcohol and put it in an oven at 80°C overnight to completely evaporate the alcohol. Adhere the structured side of the PDMS in a clean Petri dish. Vibrate the 1mg / mL nano-silver wire solution with ultrasonic vibration for 1min to distribute the nano-silver wire evenly in the whole solution, quickly use a pipette gun to add the nano-silver wire solution from the liquid inlet, so that the liquid fills the entire channel, and put it into the 80 ℃ drying in an oven, the nano-silver wires are distributed at the bottom of the entir...

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Abstract

The invention provides a preparation method of a conductive nano-silver wire patterned film. The preparation method comprises the following steps: preparing a template; uncovering cured PDMS from thetemplate for later use; adhering the face, with the structure, of a micro-fluidic chip to a culture dish or a cover slip; pouring nano-silver wires into the micro-fluidic chip, and carrying out drying; uncovering the chip; adding chitosan solution into the culture dish till the chitosan solution immerses the nano-silver wires, airing the culture dish, thus obtaining the fallen chitosan film with the patterned nano-silver wires, and placing the fallen chitosan film with the patterned nano-silver wires into NaOH solution for crosslinking, so that the stability of the chitosan film is increased.The invention further relates to applications of the conductive nano-silver wire patterned film. The prepared film is beneficial for the growth, proliferation and differentiation of nerve cells, myocardial cells and the like with the electrical signal conduction function, and the signal transmission among the cells. The prepared film has the operability and the predictably significant economic value and social value.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips and nanomaterial applications, and in particular provides a method for preparing a patterned conductive nano-silver wire film. Background technique [0002] Conductive nanomaterials have gained much attention in biomedical engineering due to their electrical conductivity, mechanical properties, and unique nanotopographic structures. Conductive nanomaterials have been recognized to have the ability to transmit electrical signals to cells. Several studies have shown that the interaction between conductive nanomaterials and cells can create electronic interfaces and alter cellular excitability within the material. Furthermore, the nanotopography of nanomaterials may enhance cell adhesion, migration and elongation to a great extent. For example, gold or silicon nanowires were observed to promote the assembly of cardiac cells, resulting in strong and anisotropic contraction forces in cardi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12Q1/02
CPCC12N5/0068C12N2533/30C12N2533/70C12N2535/10G01N33/5029G01N33/5032
Inventor 秦建华尹方超魏文博王丽
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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