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Mirabilis jalapa root general flavone extractive for preventing and curing diabetes mellitus type 2 and complication and preparation method and application thereof

A technology for type 2 diabetes and total flavonoids, which is applied in the medical field, can solve the problems of reducing drug intake, unfavorable, unexplained effective ingredients, etc., and achieves the effect of reducing tissue triglyceride content, long-lasting effect, and no toxic and side effects.

Active Publication Date: 2018-06-12
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent 102225082A submitted by the applicant is a drug for preventing and treating type 2 diabetes and its complications and its preparation method. It specifically discloses the extract of Mirabilis root, which is dried and pulverized, and then extracted with ethanol reflux, which has the ability to prevent and treat type 2 diabetes and its complications. The function of its complications, but it does not clarify the specific active ingredients. Although the extraction method is simple and direct, its extract contains other substances that are not effective for diabetes, resulting in the dosage of the extract being as high as 2-4g / kg. Not conducive to reducing drug intake

Method used

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  • Mirabilis jalapa root general flavone extractive for preventing and curing diabetes mellitus type 2 and complication and preparation method and application thereof
  • Mirabilis jalapa root general flavone extractive for preventing and curing diabetes mellitus type 2 and complication and preparation method and application thereof
  • Mirabilis jalapa root general flavone extractive for preventing and curing diabetes mellitus type 2 and complication and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] The preparation of embodiment one Mirabilis root total flavonoids extract

[0028] 1. Extraction: dried mirabilis root at 45°C-65°C, crushed mirabilis root coarse powder passed through a 20-40 mesh sieve, soaked in 8-14 times the volume of 50%-80% ethanol for 0.5-2h, Reflux extraction 1-3 times, control the reflux temperature at 60°C-80°C, 0.5h-2h each time. Filtrate and combine the extracts, concentrate with a rotary evaporator until there is no ethanol, and obtain the alcohol extract of Mirabilis root for future use.

[0029] 2. Take the macroporous adsorption resin X-5 on the concentrated liquid of the alcoholic extract of Mirabilis jasmine root. According to the static adsorption rate, static desorption rate and the maximum sample amount determined by the leakage curve of the macroporous adsorption resin X-5, select the sample loading method. Liquid concentration 8mg / ml-20mg / ml, elution rate 0.5BV / h-3BV / h, eluent composition 45%-75% ethanol, eluent volume 1BV-4BV; ...

Embodiment 2

[0031] The effect of the total flavonoids extract of embodiment 2 Mirabilis root on the blood sugar of normal and type 2 diabetic mice

[0032] In the experiment, pharmacodynamic research is carried out on the medicament of the present invention, and its influence on blood sugar in normal and type 2 diabetic (db / db) mice is observed.

[0033] 1. Experimental materials and methods

[0034]SPF-grade male C57BL / 6 mice, weighing 18-22 g, were provided by the Experimental Animal Center of the Institute of Field Surgery, Third Military Medical University. SPF grade male db / db mice were purchased from Jackson Laboratory, USA. Drinking water and feeding freely, adaptive feeding in SPF grade animal room for 1 week, temperature (20±2)°C, humidity (60±5)%, 12h photoperiod. Mirabilis root was purchased from Chongqing Traditional Chinese Medicinal Materials Market, and was identified as Mirabilis root by Chinese medicine experts and "Chinese Commonly Used Chinese Herbal Medicine" and "Ch...

Embodiment 3

[0073] Embodiment 3 The preparation of the pharmaceutical composition containing Mirabilis root total flavonoids extract

[0074] 1. Preparation of total flavonoids capsules from Mirabilis root

[0075] A Chinese herbal medicine, which is made from the root of the Chinese herbal medicine Mirabilis, and is used for treating diabetes and its complications.

[0076] Formula: total flavonoids of Mirabilis root (active ingredient) 200g, lactose (excipient) 80g, starch (excipient) 70g, magnesium stearate (anti-adhesive agent) 2.5g, made into 1000 capsules in total.

[0077] Preparation method: pass the active ingredients and pharmaceutical excipients through a 100-mesh sieve, weigh them accurately according to the prescription, mix them in a mixer for 10-15 minutes, add 2.5g of magnesium stearate, mix them for 2 minutes, and put them into 1000 capsules. have to.

[0078] 2. Preparation of total flavonoids from Mirabilis root soft capsules

[0079] A Chinese herbal medicine, which...

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Abstract

The invention relates to the field of medicine, in particular to a mirabilis jalapa root general flavone extractive for preventing and curing diabetes mellitus type 2 and a preparation method and application thereof. In the extractive, the content of general flavone is greater than 40%, when the dosage of the extractive is 100 mg / kg, the extractive has obvious effects on decreasing the content ofblood glucose, total cholesterol, triglycerides, tissue triglycerides and increasing glycogen content, prophylaxis and remedial medication have similar effects on blood glucose and lipid lowering of diabetes mellitus type 2 induced by streptozotocin, also reduce blood glucose and lipid type 2 diabetes (db / db) mice of gene knockout, and do not affect glucolipid metabolism of normal mice. Accordingto the preparation method of mirabilis jalapa root general flavone extractive, mirabilis jalapa root general flavone is separated and purified by macroporous adsorption resin, in the prepared mirabilis jalapa root general flavone extractive, the content of the general flavone is greater than 40 %, the transfer rate reaches up to 70 %, and the yield rate can reach up to 2 %.

Description

technical field [0001] The invention relates to the field of medical science, in particular to an extract of total flavonoids from Mirabilis root for preventing and treating type 2 diabetes and its complications, as well as its preparation method and application. Background technique [0002] Mirabilis jalapa L belongs to the family Mirabilis. The leaves, roots, seeds and flowers of Mirabilis can be used as medicine. It tastes sweet and bitter, and enters the kidney, liver, and bladder. It can treat acute arthritis, bruises, prostatitis, scabies, hemorrhoids and other diseases, but it is contraindicated for pregnant women. Mirabilis root, also known as ground mouse, pollen head, rouge, etc., is cultivated in most parts of the country. The roots contain amino acids, organic acids and a large amount of starch, while the flowers contain a variety of yellow pigments such as beetaxanthin. Modern pharmacological research shows that Mirabilis has anti-fertility, insecticidal, ant...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P3/10A61K125/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 周吉银刘祚刘丹
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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