Sand culture method capable of quickly screening low-cadmium grain rice varieties

Abstract

The invention discloses a sand culture method capable of quickly screening low-cadmium grain rice varieties. The sand culture method includes: preparing a culture medium-river sand; collecting river sand of 20-40 meshes, using clear water to wash the river sand, using a HCl solution of 3.8% in mass concentration for soaking for 30min, using tap water to flush, and taking cleaning liquid obtained in the last time for cadmium content testing, wherein when cadmium content is lower than 0.001mg / L, the river sand can be used for rice culture experiments; preparing sand culture liquid of 1.0mg / L, 0.5mg / L and 0.2mg / L in cadmium concentration; respectively planting to-be-screened rice varieties in culture buckets, inserting 2-4 roots in each culture bucket, and performing conventional fertilizingculture, wherein each root is for a seedling; using cadmium-pollution-free water as irrigation water; when rice is mature, harvesting rice seeds, and taking rice varieties of lower than 0.2mg / kg in rice cadmium content as low-cadmium grain rice varieties which can be further used for field experiments. The sand culture method is few in experiment error, high in repeatability, scientific and reliable in result and free of impact on biological yield of rice.

Description

technical field [0001] The invention belongs to the technical field of rice cultivation and relates to a sand culture method for quickly screening low-cadmium grain rice varieties. Background technique [0002] Cadmium (Cd) is one of the most common heavy metals polluted in farmland in my country. According to the statistics of the Ministry of Agriculture, more than 10% of the cultivated land in my country is polluted by cadmium (more than 0.3mg / Kg), and the grains polluted by heavy metals such as cadmium are as high as 12 million tons every year. Cadmium pollution enters the human body through the food chain, and long-term consumption will seriously endanger human health, and even cause "bone pain disease". [0003] Hunan is the hometown of "non-ferrous metals". Among the 31 batches of rice with excessive cadmium content detected by the Guangdong Food Safety Office in 2013, 14 batches came from Hunan, and the cadmium content ranged from 0.26mg to 0.93mg per kilogram. wait...

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Problems solved by technology

[0005] Up to now, domestic scholars have generally used field or hydroponic methods to screen rice varieties with low cadmium grains. However, due to the extremely uneven distribution of available cadmium and adsorbed cadmium in the soil, the cadmium content and pH value of irrigation water in polluted areas are variable. The results brought large errors, and the repeatability of the experiment was extremely poor; while the hydroponics method was cumbersome and complicated to operate, and hydroponics could not fix the rice well, which had a serious impact on the growth and development of rice.

Therefore, an ideal method for screening low-cadmium rice varieties in grains has not been established so far, and it is impossible to truly evaluate the cadmium uptake levels of different rice varieties under different cadmium content backgrounds, which seriously restricts the rapid screening and discovery of low-cadmium rice varieties. Rice Variety / Strain

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