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Application of specific lectin composition in preparing test carrier for identifying epithelial ovarian cancer, and kit

A lectin and ovarian cancer technology, applied in biological testing, measuring devices, instruments, etc., can solve problems such as low specificity, hazards of blood-borne diseases in samples, unsuitability for large-scale population censuses, etc., and achieve high-throughput detection, Effects that are easy to capture

Active Publication Date: 2018-06-01
深圳格道糖生物技术有限公司
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Problems solved by technology

[0009] In order to solve the current problems in the differential diagnosis of epithelial ovarian cancer, such as low specificity, hazards of blood-borne diseases in samples, and unsuitability for large-scale population census, etc.

Method used

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  • Application of specific lectin composition in preparing test carrier for identifying epithelial ovarian cancer, and kit
  • Application of specific lectin composition in preparing test carrier for identifying epithelial ovarian cancer, and kit
  • Application of specific lectin composition in preparing test carrier for identifying epithelial ovarian cancer, and kit

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Embodiment Construction

[0032] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0033] The present invention screens lectin probes for differential diagnosis of female patients with epithelial ovarian cancer from 37 lectins (as shown in Table 1).

[0034] Table 1

[0035]

[0036]

[0037]

[0038] The kit materials used in the present invention include: protease inhibitor, DMSO, Cy3, Cy5 fluorescent dyes, Sephadexg G-25 column, bovine serum albumin (BSA), glass sheet base, oxysilane reagent (GPTS), Bradford reagent , PVDF membrane, Tween-20.

[0039] 1. Collection of Saliva Samples

[0040] A total of 192 saliva samples were collected, including 63 cases of healthy female volunteers (HV), 51 cases of saliva from patients with benign ovarian diseases (OB), and 78 cases of saliva from patients with confirmed epithelial ovarian cancer (OC), including 24 cases of stage I patients. , 22 cases of stage II ...

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Abstract

The invention provides application of a specific lectin composition in preparing a test carrier for identifying epithelial ovarian cancer, and a kit. Aiming at a saliva sample, the specific lectin composition comprises (1) a composition of PSA, SNA, MAL-II, ECA and HHL for a healthy crowd; (2) a composition of PHA-E, LTL and PNA for patients with benign ovarian diseases; (3) a composition of RCA120, UEA-I, ConA, LCA, AAL, WFA, WGA, DBA, VVA, NPA and GNA for patients with epithelial ovarian diseases. Through specific combining of lectin and glycoprotein carbohydrate chain in saliva, glycoprotein and a carbohydrate chain structure in differential expression in the saliva sample of a patient can be quickly detected, and multiple glycoprotein and carbohydrate chain structures in the sample canbe quickly detected in high flux.

Description

technical field [0001] The invention relates to the use and kit of a lectin for identifying epithelial ovarian cancer based on salivary glycoprotein. Background technique [0002] Ovarian cancer (Ovarian cancer, OC) is one of the most common tumors of the female reproductive system, ranking sixth among the most common cancers in the world, fifth among common female cancers, and seventh among the major cancers that cause death in women globally . In recent years, the incidence of ovarian cancer in my country has been on the rise. It is estimated that the incidence of ovarian cancer in my country will reach 6.43 / 100,000 in 2016. Due to the lack of obvious differential signs and screening methods, early diagnosis of ovarian cancer is very difficult. Clinical studies have shown that the 5-year survival rate of ovarian cancer patients diagnosed in the early stage (I / II) can reach more than 90%, however, about 70%-85% of the patients are already in the advanced stage (III / IV stag...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57449G01N33/68
Inventor 李铮薛添张坤舒健刘夏薇阳佳君于汉杰杜昊骐
Owner 深圳格道糖生物技术有限公司
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