Enzymatic chemiluminescence based detection kit for UA (uric acid) determination

Abstract

The invention belongs to the technical field of biology and particularly relates to an enzymatic chemiluminescence based detection kit for UA (uric acid) determination. UA is oxidized into allantoin and hydrogen peroxide under catalysis of uricase, hydrogen peroxide is subjected to direct reaction with a luminescence substrate, and a persistent high-intensity chemical signal is formed without peroxidase. Compared with the traditional enzymatic colorimetry and chemiluminescence, hydrogen peroxide is subjected to direct reaction with the substrate for luminescence without need of adding catalasefor catalysis, and the method can be applied to full-automatic luminescence instruments and can meet clinical automatic batch detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for measuring uric acid by an enzyme chemiluminescence method. Background technique [0002] The molecular formula of uric acid (2,4,6-trihydroxypurine, referred to as UA) is C 5 h 4 N 4 o 3 , its molecular weight MW=168.11. It is a white crystalline substance that is insoluble in water, acid, ether and ethanol. It is weakly alkaline and can form a salt with strong acid. Uric acid exists in the form of salt in the living body, so it has a high solubility. Uric acid is the end product of purine metabolism in the human body. Most of it is excreted with urine, and a small part is excreted through feces and sweat. Normal value of plasma uric acid: male: 149-416 μmol / L, female: 89-357 μmol / L. When the purine metabolism in the human body is disturbed, the concentration of uric acid in the plasma is too high, which is called hyperuricemia (HUA). The solub...

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Problems solved by technology

However, this method uses luminol, isoluminol and their derivatives as luminescent substrates, which generally need to add catalysts such as peroxidase. The stability of these reagent components is poor, and the specificity of peroxidase The sensitivity is not high, because hydrogen peroxide is the main intermediate product in this reaction, it is easy to be interfered in the determination process

[0005] Secondly, what cannot be ignored in the process of detecting uric acid at present is the interference caused by endogenous substances in the sample such as hemoglobin, bilirubin and exogenous substances derived from clinical drug applications, such as ascorbic acid, which leads to the detection of uric acid. There is a certain difference between the result and the real value

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