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Fluid medium of oudemansiella raphanipes strain and preparation method of fluid medium

A technology of liquid culture medium and Gallus firsum, applied in botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of slow mycelial growth and low yield, and achieve mycelial growth The effect of fast speed, accelerated growth speed, and promotion of mycelial growth

Inactive Publication Date: 2018-06-01
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the problems existing in the prior art, the object of the present invention is to provide a kind of liquid culture medium and preparation method thereof that can improve the mycelia growth rate of the female species of P. Problems of slow mycelial growth and low yield during cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of liquid culture medium of the parent species of Gallus fruticosa nigricans, described culture medium is 50% improved PDA+50%DDS, and described improved PDA is potato juice 200g, grape juice 30g, soybean sprouts 90g, wheat bran 40g, agar 25g, potassium hydrogen phosphate 1g, magnesium sulfate 0.5g, yeast extract 2g, water 1000ml.

[0021] The preparation method of the liquid culture medium of above-mentioned P. nigerii female species, it may further comprise the steps:

[0022] Step 1): Beat the peeled and removed potatoes into a fine pulp according to the mass ratio, filter the fine pulp with four layers of gauze for 3 times, and take the filtrate to obtain potato juice;

[0023] Step 2): after fresh grape juice is squeezed, gauze is filtered 3 times to obtain grape juice;

[0024] Step 3): according to the mass ratio, chop the soybean sprouts, mix them with wheat bran, add an appropriate amount of water for cooking, the cooking time is 60 minutes, smash and f...

Embodiment 2

[0028] A kind of liquid culture medium of the parent species of Gallus fruticosa nigella, described culture medium is 50% improved PDA+50%DDS, and described improved PDA is potato juice 180g, grape juice 28g, soybean sprouts 85g, wheat bran 38g, agar 25g, potassium hydrogen phosphate 1g, magnesium sulfate 0.8g, yeast extract 2.5g, water 980ml.

[0029] The preparation method of the liquid culture medium of above-mentioned P. nigerii female species, it may further comprise the steps:

[0030] Step 1): Beat the peeled and removed potatoes into a fine pulp according to the mass ratio, filter the fine pulp with four layers of gauze for 3 times, and take the filtrate to obtain potato juice;

[0031] Step 2): after fresh grape juice is squeezed, gauze is filtered twice to obtain grape juice;

[0032] Step 3): according to the mass ratio, chop the soybean sprouts, mix them with wheat bran, add an appropriate amount of water for cooking, the cooking time is 80 minutes, break up after...

Embodiment 3

[0036] A kind of liquid culture medium of the parent species of Gallus fruticosa nigricans, described culture medium is 50% improved PDA+50%DDS, and described improved PDA is potato juice 160g, grape juice 25g, soybean sprouts 80g, wheat bran 36g, agar 24g, potassium hydrogen phosphate 0.8g, magnesium sulfate 1g, yeast extract 2.2g, water 960ml.

[0037] The preparation method of the liquid culture medium of above-mentioned P. nigerii female species, it may further comprise the steps:

[0038] Step 1): Beat the peeled and removed potatoes into a fine pulp according to the mass ratio, filter the fine pulp with four layers of gauze for 3 times, and take the filtrate to obtain potato juice;

[0039] Step 2): after fresh grape juice is squeezed, gauze is filtered 3 times to obtain grape juice;

[0040] Step 3): According to the mass ratio, chop the soybean sprouts, mix them with wheat bran, add an appropriate amount of water for cooking, the cooking time is 70 minutes, smash and ...

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Abstract

The invention discloses a fluid medium of an oudemansiella raphanipes strain. The medium is combination of 50% improved PDA and 50% DDS, wherein the improved PDA consists of 150-200g of tomato juice,25-30g of grape juice, 80-100g of soybean sprouts, 30-50g of wheat bran, 20-25g of agar, 0.5-1g of potassium hydrogen phosphate, 0.5-0.8g of magnesium sulfate, 1.5-2.5g of a yeast extract and 800-1000ml of water. The preparation method of the fluid medium of the oudemansiella raphanipes strain comprises the following steps: 1) preparing the tomato juice; 2) preparing the grape juice; 3) preparinga mixed solution of soybean sprouts and wheat bran; and 4) in accordance with component proportions, uniformly mixing the various components, so that the fluid medium is obtained. The fluid medium provided by the invention, which adopts the improved PDA and the DDS, can offer various nutritional substances required by growth of oudemansiella raphanipes myceliae, so that the growth speed of the oudemansiella raphanipes myceliae can be effectively accelerated.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a liquid culture medium of the female species of Gallus fruticosa and a preparation method thereof. Background technique [0002] Gallopia black skin belongs to the order Agaricaceae, and it is a kind of wild edible fungus that grows in summer and autumn in temperate and subtropical regions. Because its growth and development are closely related to the activities of soil termites, and it is highly dependent on the growth area, the artificial cultivation requires high and difficult requirements. And because of the tender meat, strong smell and delicious taste, it is one of the famous wild edible mushrooms, and the taste of the black-skinned mushroom is the best. Traditional Chinese medicine believes that the fungus is flat and sweet, has the effects of invigorating the spleen and Qi, appetizing and refreshing, and relieving pain and edema. It is an i...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G18/20C12R1/645
CPCC12N1/14
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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