SCAR-PCR identification method for mating types of agaricus bisporus homonuclear sterile single spore strains

Abstract

The invention belongs to the field of edible mushroom molecular marker assistant breeding, and particularly relates to an SCAR-PCR identification method for the mating types of an agaricus bisporus homonuclear sterile single spore strains. In the method, an SCAR molecular mark is utilized for performing PCR specific amplification on the agaricus bisporus homonuclear sterile strains with differentmating types. In the method, a complex hybridizing pairing experiment is not needed, and the mating types of the agaricus bisporus homonuclear sterile single spore strains are identified specifically.The method is short in consumed time, easy and convenient to operate and high in specificity. According to existence of a specific amplification product 1283bp band, the sterile single spore strainswith the mating type A+ are identified, and according to existence of a specific amplification product 860bp band, the sterile single spore strains with the mating type A- are identified. With the method, in agaricus bisporus hybridizing breeding, the mating types of the homonuclear sterile strains can be rapidly identified, and therefore the efficiency of hybridizing breeding is greatly improvedfor agaricus bisporus.

Description

technical field [0001] The invention belongs to the field of molecular marker assisted breeding of edible fungi, and more specifically relates to a SCAR-PCR identification method for the mating type of Agaricus bisporus iso-sterile monosporus strain. Background technique [0002] Agaricus bisporus belongs to the bipolar secondary homothallic edible fungus. Most basidiospores are heterokaryotic and cannot be used for hybridization. Only homokaryotic strains germinated by homokaryotic spores can be used for hybridization. We apply isoenzymes or DNA fingerprints to distinguish and identify heterokaryon and homokaryon at the hyphae stage, which greatly improves the efficiency of hybrid breeding of Agaricus bisporus. However, different homokaryote strains still have the same or different mating types. Only homokaryotes with different mating type factors can pair successfully, and the success rate of random pairing to obtain hybrids is not high enough. Previous studies have shown...

AI Technical Summary

Problems solved by technology

Although the genome sequence of Agaricus bisporus was published in 2010, the sequence of factor A has not been reported

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