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Method for improving salt tolerance of plants

A plant stress tolerance, plant technology, applied in the direction of botanical equipment and methods, biochemical equipment and methods, plant peptides, etc., to achieve the effect of improving salt tolerance

Inactive Publication Date: 2018-05-25
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Plant salt tolerance is a quantitative trait that is controlled by multiple genes, but the improvement of plant salt tolerance is currently limited to the study of a single gene

Method used

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  • Method for improving salt tolerance of plants
  • Method for improving salt tolerance of plants
  • Method for improving salt tolerance of plants

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Experimental program
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Embodiment 1

[0050] Embodiment 1, the acquisition of transgenic Arabidopsis and its salt tolerance analysis

[0051] 1. Obtaining transgenic Arabidopsis

[0052] 1. Construction of the carrier

[0053] (1) Double gene plant expression vector

[0054] Clone hippocampal tooth cell membrane Na + / H + antiporter gene SpSOS1 and membrane H + -ATPase gene SpAHA1, and construct it into the same plant expression vector pCAMBIA1304. The specific operation is as follows: first clone the SpSOS1 gene (sequence 1), and insert it between the Sal I and Kpn I sites of the pCAMBIA1300 vector (Cambia, Australia) to obtain the starting plasmid pCAMBIA1300-SpSOS1; I double-digest plasmid pCAMBIA1300-SpSOS1, and insert the fragment (which contains 35S promoter, SpSOS1 gene and NOS terminator) into pCAMBIA1304 vector (Cambia, Australia) to obtain intermediate plasmid pCAMBIA1304-SpSOS1; finally clone SpAHA1 gene (sequence 3), and it is inserted between the Spe I of pCAMBIA1304-SpSOS1 carrier and the Eco72...

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Abstract

The invention discloses a method for improving salt tolerance of plants. In the method for improving the salt tolerance of the plants, cell membrane Na+ / H+ reverse transport protein gene SpSOS1 and anH+-ATPase gene SpAHA1 are co-transformed into the plants, and the expression quantity and / or activity of SpSOS1 protein and SpAHA1 protein in the receptor plants is improved. Experiments prove that with the SpSOS1 protein and SpAHA1 protein, which is concretely embodied in that the salt tolerance of the plants can be improved, and the fresh weight, the root length, the number of lateral roots, the flowing speed of H+ ions, the flowing speed of Na+ ions and the content of K+ of the plants are increased, and the content of Na+ and malondialdehyde is decreased. With the method, the cell membraneNa+ / H+ reverse transport protein gene SpSOS1 and an H+-ATPase gene SpAHA1 are co-transformed into the plants to prove the salt tolerance for the first time, which directly proves that H+-ATPase can provide energy for an Na+ / H+ reverse transport system.

Description

technical field [0001] The invention relates to the field of plant transgenic breeding, in particular to a method for improving salt tolerance of plants. Background technique [0002] According to the different salt tolerance of plants, plants can be divided into halophytes and light-soil plants. Halophytes can grow normally and complete their life cycle in a high-salt environment (salt content exceeds 1%). Therefore, the study of the salt tolerance mechanism of halophytes can not only make full use of various salinized lands, but also provide theoretical guidance for improving the salt tolerance of light soil plants. Seahorse tooth (Sesuvium portulacastrum L.) is a halophyte that grows in the sea. In a medium saline environment, Na + It can promote the succulent of hippocampus tooth leaves and the development of seedlings. Therefore, hippocampus tooth is a very good material for studying the mechanism of plant salt tolerance. [0003] Na on the cell membrane under salt s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 周扬夏幽泉江行玉范亚飞
Owner HAINAN UNIVERSITY
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