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Method for evaluating plant DNA damage of radioactive gas radon by using usnea diffracta

A radioactive gas and DNA damage technology, applied in the preparation of test samples, measuring devices, sampling, etc., can solve problems such as the difficulty of separating nuclei

Inactive Publication Date: 2018-05-22
QINGDAO AGRI UNIV
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Problems solved by technology

However, in previous studies, the research object of the comet experiment mainly focused on the detection of DNA damage in animal cells and human cells. This is due to the obvious difference in the cell structure of plants and animals: plant cells have strong cell walls, causing Difficulty in isolating cell nuclei, thus limiting the application of comet assay techniques in the field of plants

Method used

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  • Method for evaluating plant DNA damage of radioactive gas radon by using usnea diffracta
  • Method for evaluating plant DNA damage of radioactive gas radon by using usnea diffracta
  • Method for evaluating plant DNA damage of radioactive gas radon by using usnea diffracta

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Embodiment

[0030] 1. Putting radon in plant samples

[0031] Choose Usnea as the experimental material. Because Usnea is an epiphyte, it does not need to grow in the soil and is in a clump shape. Therefore, weigh 3 clumps of healthy Usnea with a balance, each 200g, and divide them into a control group (the effect of the control group is shown in Table 1, and the degree of damage can be compared only if there is a control group) and two experimental groups. The control group was placed in a normal environment (the radon concentration was about 20Bq / m 3 ), the experimental group was placed at 1m 3 Treatment was carried out in the radon chamber, the initial exposure concentrations were 3910 and 2530Bq / m 3 . Evenly hang the usnea in a fixed position in the radon chamber with thin wires. Through the radon source ( 226 Ra) is charged with radon gas, the concentration of radon in the room is increased to the initial concentration, and the supply of radon is stopped, so that the plants are...

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Abstract

The invention discloses a method for evaluating plant DNA damage of a radioactive gas radon by using usnea diffracta. The method comprises the following steps: putting usnea diffracta into a radon chamber, extracting cell nucleuses after radon treatment is completed, further performing single cell gel electrophoresis testing, measuring parameters of DNA migration by using a CASP (Critical Assessment of Techniques for Protein Structure Prediction) system, and evaluating DNA damage degrees. By adopting the method, the usnea diffracta is adopted as a plant material for a first time to reflect plant DNA damage; with the combination of methods of mechanical separation and enzyme treatment, cell nucleuses in stems and leaves of plants are separated, then an appropriate number of complete nucleuses are obtained, and the problem that comet assay is hard to apply to the plant field is solved; the Tail length, the relative Tail DNA content, the tail momentum (TM) and the Olive tail momentum (OTM) which can reflect plant DNA damage degrees are confirmed, and relatively rich bases can be made for researching functions of radon to organisms.

Description

technical field [0001] The invention belongs to the technical field of air pollutant toxicity evaluation, and in particular relates to an evaluation method for plant DNA damage caused by radioactive gas radon. Background technique [0002] radon( 222 Rn) is uranium ( 238 U) in the decay chain of radium ( 226 Ra) decay product with a half-life of 3.82d. because 226 Ra widely exists in various geological environments such as rocks and soils, so radon widely exists in the atmosphere and is the most important natural radioactive gas in nature. The accumulation of radon in human lungs can easily lead to lung cancer and other cancers, which has attracted great attention from governments and people all over the world. The International Agency for Research on Cancer (IARC) has classified radon and its progeny as a class I carcinogen, and the World Health Organization (WHO) also regards radon as one of the 19 human carcinogens. [0003] The damage of radon and other radioactive...

Claims

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Application Information

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IPC IPC(8): G01N27/447G01N1/34G01N1/28
CPCG01N1/286G01N1/34G01N27/447G01N2001/2866
Inventor 李鹏郑桂灵
Owner QINGDAO AGRI UNIV
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